用单孢子接种,分别通过固体平板培养与液体培养,测定氟对分离自黑茶的6株冠突散囊菌和1株黑曲霉生长的抑制率、菌体生长速率和菌丝生物量,研究氟对各菌株生长的影响。结果表明：6株冠突散囊菌在含氟2 000 mg/L、黑曲霉在含氟1 800 mg/L的固体培养基中能生长。100 mg/L剂量的氟对冠突散囊菌生长的抑制率为0.76%~10.24%,对黑曲霉的抑制率达35.71%。随氟浓度的增加,生长速率变小,抑制率增大。液体培养中,100 mg/L的氟对冠突散囊菌与黑曲霉菌丝生物量的抑制率分别为34.47%、80.59%。冠突散囊菌的子囊孢子与黑曲霉的分生孢子对氟具有较强的耐受性,且两种培养方式表明冠突散囊菌比黑曲霉更耐氟。

Six strains of Eurotium Cristatum fungi marked GF1~GF6 and a strain of Aspergillus niger fungi were isolated from several kinds of dark tea. Preliminary identification of the strains was done by morphology. For the purpose of revealing the influence of fluorine on the six strain of Eurotium Cristatum and one strain of Aspergillus Niger fungi, the growth inhibition rates, mycelial growth rates and mycelial biomass in the treatment of different dosages of fluorine were measured on the plate culture and liquid culture inoculated with single spore. The results showed that six strains of Eurotium Cristatum could grow in 2 000 mg/L fluorine, and Aspergillus niger strain could grow in 1 800 mg/L fluorine under plate culture condition. The growth inhibition rates of Eurotium Cristatum strains ranged from 0.76% to 10.24% while the Aspergillus niger was 35.71% under the dosage of 100 mg/L fluorine. The inhibition rate became higher as the dosage increased. However, the inhibition was more significant under liquid culture condition. The inhibition rate of mycelial biomass of strain GF3 and Aspergillus niger strain under the dosage of 100 mg/L were 34.47%, 80.59% respectively. Ascospore of Eurotium Cristatum and conidia of Aspergillus niger fungi showed more tolerant to fluorine. Also, Eurotium Cristatum strains were more tolerant to fluorine than Aspergillus niger strain both on plate culture and liquid culture.