建立茶树品种分子指纹图谱对茶树资源鉴别、品种权益保护、苗木纯度检测等具有重要意义。采用17个SSR标记对湖南省主要茶树品种尝试构建了分子指纹图谱,提出了构建茶树分子身份证的方法模式。17对SSR引物共检测出41个等位位点,每个引物检测到的等位位点变化范围为2~3个,平均2.4个。根据茶树SSR标记带型特点,将扩增得到的1、0数据进行了基因型转换,分别用1、2、3、4……N来代表不同的基因型,构建了一套湖南省主要茶树品种的分子指纹图谱,使每个品种都获得了一个17位数的指纹图谱号码,进而可将参试品种完全区分开。同时,对参试品种进行了特异性指数分析,品种特异指数介于65.4~113.7之间,平均80.1,表明品种间的特异性差异较大。
Molecular fingerprints is very important to identify new varieties, cultivars authenticity and seedings purity. Seventeen pairs of SSR primers were used to construct the molecular fingerprints of tea varieties originated from Hunan province. Totally 41 alleles were amplified using the 17 SSR primers, the number of alleles per primer ranged from 2 to 3, with an average of 2.4. The SSR alleles were determined for bands size(bp) and scored as present(1) or absent (0). According to the genotypes code rule, the 1 and 0 results which one pair of SSR primers amplified in a variety could be converted to genotypes code of 1, 2……N following their fragment size. The results proved it’s a very useful tool for tea resource appraising, all accessions could be discriminated from a set of molecular fingerprints. Variety speciality index varied from 65.4 to 113.7, averagely 80.1, which indicated that there is great difference in speciality among all accessions.
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