利用普洱茶加工过程中废弃的碎茶灰为原料制备提取物,分析其主要化学成分组成,以ABTS和FRAP体系评价了普洱生茶、普洱熟茶提取物的抗氧化活性,并研究了这两种提取物对Na2S2O3诱导人脐静脉血管内皮细胞（HUVEC）损伤的保护作用。结果表明,普洱熟茶提取物中主要化学成分除咖啡碱外均低于普洱生茶提取物,其中酯型儿茶素含量减少最多。同时,普洱熟茶提取物对ABTS+清除能力和铁离子还原能力都较普洱生茶提取物作用弱,而对HUVEC损伤的保护作用却强于普洱生茶提取物,并在特定浓度下与茶多酚相当。
Extracts of raw and ripe Pu-erh tea were prepared from tea dust during processing. The antioxidtive activities of extracts were evaluated by ABTS and FRAP systems. The potentially protective effects of extracts on human umbilical vascular endothelial cells(HUVEC) were investigated in Na2S2O3-induction model. The results showed that the extract of raw Pu-erh tea had stronger antioxidtive activity than that of ripe Pu-erh tea in both test systems. But extract of ripe Pu-erh tea showed a less protective effect than that of extract of raw Pu-erh tea on the HUVEC cell damage induced by Na2S2O3, and relevent with tea polyphenols under certain concentrations.
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