茶黄素双没食子酸酯的抗癌活性及其作用机理研究

江和源; HangXiao; 袁新跃; 王川丕; Chung S Yang

doi:10.13305/j.cnki.jts.2007.01.005

茶叶科学 >

2007 , Vol. 27 >Issue 1: 33 - 38

DOI: https://doi.org/10.13305/j.cnki.jts.2007.01.005

茶黄素双没食子酸酯的抗癌活性及其作用机理研究

江和源 ,
HangXiao ,
袁新跃 ,
王川丕 ,
Chung S Yang

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1. 中国农业科学院茶叶研究所,浙江杭州 310008;
2. Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ08854, USA.

江和源(1974— ),男,江西都昌人,工学博士,现从事茶叶天然产物提取与利用、茶叶化学与加工等研究。

收稿日期: 2006-07-27

修回日期: 2006-09-27

网络出版日期: 2019-09-11

基金资助

国家自然科学基金（30570191）和浙江省自然科学基金（Y304477）研究内容

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Anticancer Activity of Theaflavin Diagallate and its Mechanism

JIANG He-yuan ,
Hang Xiao ,
YUAN Xin-yue ,
WANG Chuan-pi ,
Chung S Yang

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1. Tea Research Institute, Chinese Academy of Agriculture Sciences, Zhejiang, Hangzhou 310008, China;
2. Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ08854, USA

Received date: 2006-07-27

Revised date: 2006-09-27

Online published: 2019-09-11

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摘要

以Sephadex LH-20柱色谱分离得到茶黄素双没食子酸酯（TFDG）成分,以H1299和HCT-116细胞分析了该成分的抗癌活性及其作用机理。结果表明,TFDG具有良好的抑制H1299细胞生长的活性,IC₅₀为25μmol/L;有调节细胞周期的活性,可增加HCT-116细胞G1期细胞的比例;有促进HCT-116细胞凋亡的作用,浓度为50μmol/L时效果显著,48βh凋亡率达到40%以上;Western杂交技术分析结果表明,它可降低HCT-116细胞中促癌蛋白质因子Bcl-xL的表达量,可增加抑癌蛋白质因子Bax的表达量。

关键词： 茶黄素; 抗癌; 细胞周期; 凋亡; 蛋白质印迹

本文引用格式

江和源 , HangXiao , 袁新跃 , 王川丕 , Chung S Yang . 茶黄素双没食子酸酯的抗癌活性及其作用机理研究[J]. 茶叶科学, 2007 , 27(1) : 33 -38 . DOI: 10.13305/j.cnki.jts.2007.01.005

Abstract

Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC₅₀ value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line.

Key words： theaflavin; anticancer; cell cycle; apoptosis; western blotting

参考文献

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