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基因工程菌生物合成茶氨酸条件研究

  • 王丽鸳 ,
  • 王贤波 ,
  • 成浩 ,
  • 周健
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  • 农业部茶叶化学工程重点实验室,中国农业科学院茶叶研究所,浙江 杭州 310008
王丽鸳(1978–),女,助理研究员,主要从事茶叶生物技术研究。* 现在工作单位是杭州市农业科学院实验中心。

收稿日期: 2006-09-28

  修回日期: 2007-02-07

  网络出版日期: 2019-09-11

Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli

  • WANG Li-yuan ,
  • WANG Xian-bo ,
  • CHENG Hao ,
  • ZHOU Jian
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  • Key Lab of Tea Chemical Engineering, Ministry of Agriculture; Tea Research Institute, Chinese Acade my of Agricultural Sciences, Hangzhou 310008, China

Received date: 2006-09-28

  Revised date: 2007-02-07

  Online published: 2019-09-11

摘要

研究了不同诱导条件对基因工程菌催化合成茶氨酸的影响,确定了较为优化的诱导表达条件;通过单因子试验研究了不同反应条件对基因工程菌催化合成茶氨酸的影响,研究结果表明反应体系中较高的菌体浓度对催化茶氨酸有一定的抑制作用;高L-Gln浓度可以提高茶氨酸的生成量,但是却降低了L-Gln的转化率;基因工程菌催化合成茶氨酸的最适pH是9.5左右,较为适合的反应温度是32℃~37℃。

本文引用格式

王丽鸳 , 王贤波 , 成浩 , 周健 . 基因工程菌生物合成茶氨酸条件研究[J]. 茶叶科学, 2007 , 27(2) : 111 -116 . DOI: 10.13305/j.cnki.jts.2007.02.003

Abstract

Expression conditions of the genetic engineering E.coli which expressed E.coli γ-GGT were investigated. The optimum IPTG concentration and inductive temperature were determined. Factorial experiment design was employed to determine the effects of the reactive conditions (such as microbe concentration, pH, substitute concentration, reactive temperature) on theanine biosynthesis. Results showed that theanine biosynthesis was inhibited by the high microbe concentration. By the higher concentration of L-Gln, the more theanine was obtained and the convertion rate against Gln was declined. It also was manifested that pH9.5 and 32℃~37℃ were optimum conditions for theanine biosynthsis.

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