对咖啡碱（CAF）和牛血清白蛋白（BSA）在双酯型茶黄素形成中的影响进行了研究分析。结果表明,咖啡碱和牛血清白蛋白对TFDG的形成均有明显的抑制作用,而且相同条件下,牛血清白蛋白和咖啡碱表现出相似的抑制作用。当牛血清蛋白与咖啡碱同在一个化学氧化反应体系中时,表现出随浓度不同而呈现复杂的竞争或协同作用。
The impact of caffeine(CAF) and bovine serum albumin (BSA) on the formation of theaflavin-3,3’-digallate was compared and analyzed. The results on chemical oxidation showed that CAF and BSA had obvious inhibitory action on the formation of Theaflavin-3,3’-digallate. Under identical condition, BSA showed the same inhibitory action as CAF. When BSA and CAF were existing in the same chemical oxidation system, the complicated competition and cooperation were investigated.
[1] Roberts E A H. The phenolic substances of f manufactured tea(I)[J]. J Sci Food Agric, 1957, 8: 72~80.
[2] Erba D, Riso P, Foti P, et al. Black tea extract supplementation decreases oxidative damage in Jurkat T cells[J]. Arch Biochem Biophys, 2003, 416(2): 196~201.
[3] Babich S M, Pinsky E T.In vitro cytotoxicity of a theaflavin mixture from black tea to malignant, immortalized, and normal cells from the human oral cavity[J]. Toxicology in Vitro. 2006, 20(5): 677~688.
[4] Leung K, Su Y, Chen R, et al. Theaflavins in black tea and catechins in green tea are equally effective antioxidants[J]. Journal of Nutrition, 2001, 131(9): 2248~2251.
[5] Lu Jiebo, G hai, G etha, et al. Differential effects of theaflavin monogallates on cell growth, apoptosis, and Cox-2 gene expression in cancerous versus normal cells[J]. Cancer Research, 2000, 60(22): 6465~6471.
[6] Min-Hsiung P.Suppression of lip polysaccharide- induced nuclear factor-κB activity by theaflavin-3,3’-digallate from black tea and other polyphenols through down-regulation of IκB kinase activity in macrophages[J]. Biochemical Pharmacology, 2000, 59(4): 357~367.
[7] Thomas Muthumani, R S Senthil Kumar. Influence of fermentation time on the development of compounds responsible for quality in black tea[J]. Food Chemistry, 2007, 101(1): 98~102.
[8] Saha P, Das S.Elimination of Deleterious Effects of Free Radicals in Murine Skin Carcinogenesis by Black Tea Infusion, Theaflavins & Epigallocatechin Gallate[J]. Asia Pac J Cancer Prev, 2002, 3(3): 225~230.
[9] Saha P, Das S.Regulation of hazardous exposure by protective exposure: modulation of phaseⅡdetoxification and lipid peroxidation by Camellia sinensis and Swertia chirata[J]. Teratog Carcinog Mutagen, 2003: 313~322.
[10] Basu S T, Chaudhuri. The theaflavin fraction is responsible for the facilitatory effect of black tea at the skeletal myoneural junction[J]. Life Science, 2005, 76(26): 3081~3088.
[11] Trina Kundu, Subhabrata Dey.Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin[J]. Cancer Letters, 2005, 230(1): 111~121.
[12] Liu S H, Lu. Corrigendum to “Theaflavin derivatives in black tea and catechin derivatives in green tea inhibit HIV-1 entry by targeting gp41”[J]. Biochim. Biophys. Acta, 2005, 17(23): 270~281.
[13] 江和源, 程启坤, 杜琪珍, 等. 红茶中的茶黄素[J]. 中国茶叶, 1998, 20(3): 18~20.
[14] 谢笔钧, 石煌, 胡慰望. 绿茶、乌龙茶、红茶中主要组分和酚类化合物抑制脂肪氧合酶活性合抗油脂自动氧化特性的研究[J]. 天然产物研究与开发, 1994, 6(4): 20~23.
[15] 曲文娟, 马海乐. 茶黄素形成机理、制备与分析方法的研究进展[J]. 食品工业科技, 2006, 27(6): 197~201.
[16] 李大祥, 宛晓春, 杨昌军, 等. 茶儿茶素氧化机理[J]. 天然产物研究与开发, 2006, 18: 171~181.
[17] 王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶叶科学, 2004, 24(1): 53~599.
[18] Haslam E.Plat Polyhenils Vegetable Tannis Revisited[M]. Cambridge University Press, 1989, 10: 170.
[19] 石碧, 何先旗, 张敦信, 等. HS糅剂的研制和应用[J]. 中国皮革, 1993, 22(6): 15~17.
[20] 王泽农. 茶叶生化原理[M]. 北京: 农业出版社, 1981.
[21] 宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2003.
浙公网安备 33019902000101号 
