采用AFLP技术,选用分辨能力强、多态性高的5对引物组合对34个凤凰单丛古茶树资源进行遗传多样性分析。结果表明,5对引物共扩增出438条带,平均每对引物扩增87.6条带,多态性条带348条,多态性频率为79.3%。34个凤凰单丛古茶资源之间遗传距离的变异范围在0.13~0.49之间,平均为0.33。其中大庵宋茶与棕蓑挟之间的遗传距离最低,为0.13,字茅黄栀香与福南蜜兰、白叶单丛与通天香之间的遗传距离最大,均为0.49。构建的聚类图表明,34个凤凰单丛古茶树资源的遗传关系和它们的生物学特性和香味类型没有必然的联系。
Genetic diversity on 34 Fenghuang-Dancong tea plants were evaluated by AFLP fingerprinting using 5 primer combinations. The 5 primer combinations generated 438 bands and 87.6 bands were amplified by each primer pair on average, 348 bands(79.3%) polymorphic AFLP markers were detected, The result indicated that the genetic distance(GD) based on AFLP data by using DPS2000 was significant. The varience range of GD among the 34 Fenghuang-Dancong was 0.13~0.49. The GD(0.49) is highest between Zimaohuangzhixiang and Funanmilan and between Baiyedancong and Tongtianxiang, however, the GD(0.13) between Da-ansongcha and Zongsuojia is low. The dendrogram indicated that the genetic of 34 Fenghuang-Dancong germplasms have no certain relation with the aroma type.
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