采用MTT法及Comet assay(彗星实验分析方法)研究了表没食子儿茶素没食子酸酯（EGCG）与卡莫司汀联用对人肺癌细胞A549的生长抑制率及DNA损伤的影响,结果表明：联合应用EGCG能降低卡莫司汀对人肺癌细胞A549的IC50。卡莫司汀单用对人肺癌细胞A549的IC50为187.46βμg/ml,当用无毒浓度25βμg/ml和50βμg/ml的EGCG与卡莫司汀联用时,卡莫司汀对人肺癌细胞A549的IC50分别下降为139.56βμg/ml和154.02βμg/ml。EGCG还能增强卡莫司汀引起的人肺癌细胞A549 DNA的损伤,彗星尾矩值由单用时的19.02±14.60上升为联用时的33.07±10.47。结果说明EGCG能增强卡莫司汀对人肺癌细胞A549造成的DNA损伤,从而增强其抗肿瘤活性。
The effect of (-)-epigallocatechin gallate (EGCG) combined with carmustine on the growth inhibition and DNA damage of human lung carcinoma cell line A549 were evaluated by MTT assay and Comet assay repectively. The results showed that 50% inhibitory concentration (IC50) of carmustine to A549 human lung carcinoma cell line was decreased when combined with EGCG. The 50% inhibitory concentration (IC50) of carmustine alone to human lung carcinoma cell line A549 was 187.46βμg/ml, When the carmustine is combine with EGCG(25βμg/ml and 50βμg/ml, the concentration under which EGCG has no growth inhibition to A549), however, the IC50 value to A549 cell line decreased to 139.56βμg/ml and 154.02βμg/ml respectively. EGCG also enhanced carmustine-induced DNA damage in A549 cell line by Comet assay. The tail moment increased from 19.02±14.60 to 33.07±10.47. So, EGCG can enhance carmustine-induced DNA damage in A549 cell line, hence enhance the anticancer activity of carmustine.
[1] 邱志群, 舒为群, 田怀军, 等. 绿茶浸出液对水中有机污染物致DNA损伤的抑制作用[J]. 环境与健康杂志, 2003, 20(5): 270~272.
[2] Lynnette R Ferguson.Role of plant polyphenols in genomic stability[J]. Mutation Research, 2001, 475: 89~111.
[3] Meeran SM, Sudheer K, Mantena, et al. (-)-Epigallocatechin-3-gallate prevents photocarcinogenesis in mice through interleukin-12-dependent DNA repair[J]. Cancer Res, 2006, May 15;66(10): 5512~5520.
[4] 田士强, 张庆殷, 吴承远, 等. 替尼泊苷和卡莫司汀对脑胶质瘤的治疗作用及耐药的研究[J]. 北京医学, 2002, 24(5): 336~338.
[5] 陈科. EGCG的特性及抗肿瘤作用[J]. 美国中华临床医学杂志, 2006, 8(2): 238~239.
[6] 张星海, 唐德松, 沈生荣. EGCG防癌抗癌的药理药效研究进展[J]. 茶叶, 2001, 27(4): 43~48.
[7] 梁钢, 张肃, 黄志明, 等. ECG、EGCG对两株耐药肿瘤细胞的细胞毒增敏作用研究[J]. 中国天然药物, 2004, 2(2): 119~122.
[8] 赵玉芳, 沈生荣, 凌备备. EGCG对DNA辐射损伤的保护作用[J]. 茶叶科学, 1995, 15(2): 145~148.
[9] Takashi Kuzuhara, Yoshihisa Sei, Kentaro Yamaguchi, et al. DNA and RNA as New Binding Targets of Green Tea Catechins[J]. The Journal of Biological Chemistry, 2006, 281(25): 17446~17456.
[10] Daniela Erba, Patrizia Riso, Anna Colombo, et al. Supplementation of Jurkat T Cells with Green Tea Extract Decreases Oxidative Damage Due to Iron Treatment[J]. J Nutr,1999(129): 2130~2134.
[11] Robert F, Anderson, Louisa J, Fisher, et al .Green tea catechins partially protect DNA from ·OH radical-induced strand breaks and base damage through fast chemical repair of DNA radicals[J]. Carcinogenesis, 2001 ,(22): 1189~1193.
[12] Qing Huang, Li-Jun Wu, Shin-ichi Tashiro, et al.(+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest[J]. Journal of Pharmacological Sciences2005 (98): 16~24.
[13] Sinha D, Bhattacharya RK, Siddiqi M, et al. Amelioration of sodium arsenite-induced clastogenicity by tea extracts in Chinese hamster v79 cells[J]. J Environ Pathol Toxicol Oncol, 2005(24): 129~140.
[14] 陆瑞芳. 茶提取物与抗癌的实验研究[J]. 自然杂志, 1999, 21(6): 335~339.
浙公网安备 33019902000101号 
