研究一种从生产茶多酚后的残留液中提纯茶氨酸的制备工艺;首先通过ZTC-II型天然澄清剂对茶多酚生产废液絮凝处理,离心液经过三种规格中空超滤膜超滤,透过液用一种特制的弱极性大孔树脂（JAD-2000）初步分离,制备含量在60％以上的茶氨酸粗品,再通过C18制备柱对其溶液进行分离纯化,制备含量在98％以上高纯度茶氨酸。结果表明经过絮凝和膜超滤处理,蛋白质和果胶等杂物去除率分别达到97%、89%以上,而茶多酚、可溶糖去除率不到9%,茶氨酸保留率达到93%;JAD-2000初步分离可得含量在61％以上粗茶氨酸,回收率达到71％以上,而醇洗馏份中茶氨酸含量甚微。经C18柱制备色谱分离粗茶氨酸样液,收集12.6~15.8βmin高浓度段馏份,检测知茶氨酸的回收率为71.3%,茶氨酸含量达98.3%。
The preparative technology of theanine from the aqueous phase of tea polypheonls production was investigated. Firstly, retreating the aqueous liquids with ZTC-II, then, Separating the clarifying liquids by three kinds of ultra-filtration, the crude theanine (>60%) can be obtained from the filtrated liquid by using the JAD-2000 which is a kind of macroporous resin with weak polarity. High purity theanine (>98%) can be obtained through preparative high performance liquid chromatography (prep-HPLC) with C18 column. Results showed that proteins and pectin compounds were removed over 97% and 89% respectively, after flocculating and ultra-filtration. The removing rate of tea polypheonls and soluble sugar was less than 9% and the theanine was reserved above 93%. Crude theanine and high purity theanine were produced through JAD-2000 and the C18 column for prep-HPLC, the final recovering rate and purity of theanine were 71.5%, 61.2% and 71.3%, 98.3% respectively.
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