用mRNA差别显示技术（DDRT-PCR）研究了茶树温敏突变体——安吉白茶正常叶片和白化叶片的基因表达差异。从58个差异表达的片段中通过半定量RT-PCR鉴定出12个阳性片段,其中5个片段在正常叶片中特异表达,4个在白化叶片中特异表达,1个在绿色叶片中上调表达,2个在白化叶片中上调表达。通过与GenBank BLASTX比对分析,5个基因片段比对出相似序列,分别为拟南芥的血红素结合蛋白家族中心区域基因、甜菜的甲硫氨酸合酶基因、苜蓿的一个逆转座子基因、人类20号染色体上的一段3-磷酸甘油醛脱氢酶假基因和玫瑰的ACC合成酶基因,其余7个片段未比对上同源序列,可能为新基因。
Differences of gene expression between normal and albino leaves of tea plant (Camellia sinensis cv. Baiye1) were studied by mRNA differential display reverse transcription-PCR (DDRT-PCR). Fifty-eight differentially expressed cDNA fragments were obtained from normal and albino leaves. Twelve of which were confirmed by the analysis of RT-PCR. Among them, 5 fragments were specially expressed in normal leaves, 4 fragments were specially expressed in albino leaves, 1 fragment was up-regulated in normal leaves, and 2 fragments were up-regulated in albino leaves. Through BLASTX analysis with GenBank, 5 cDNA fragments showed high homologous to five known genes, namely soul heme-binding family protein of Arabidopsis thaliana, methionine synthase of Beta vulgaris, gag-pol polyprotein-related in Medicago truncatula, human glyceraldehyde-3-phosphate dehydrogenase pseudogene and ACC synthase of rose, respectively. The other 7 might be novel genes, they showed poor homologous to all proteins in the database.
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