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几种农杆菌的耐酚性能比较与驯化研究

  • 仓梅芹 ,
  • 周健 ,
  • 成浩 ,
  • 黎星辉 ,
  • 王丽鸳
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  • 1. 南京农业大学茶叶科学研究所,江苏 南京 210095;
    2. 中国农业科学院茶叶研究所,浙江 杭州 310008
仓梅芹,(1982— ),女,江苏盐城人,硕士研究生,主要研究茶叶生物技术。

收稿日期: 2008-01-11

  修回日期: 2008-03-12

  网络出版日期: 2019-09-16

基金资助

浙江省科技计划项目(2006C22070)、教育部高校博士点基金(20060307024)、江苏省科技攻关计划(BE2007301)

Studies on Polyphenol-resisting Characteristics of Several Kinds of Agrobacterium and the Demestication of Polyphenol-resisting Strain

  • CANG Mei-qin ,
  • ZHOU Jian ,
  • CHENG Hao ,
  • LI Xing-hui ,
  • WANG Li-yuan
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  • 1. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received date: 2008-01-11

  Revised date: 2008-03-12

  Online published: 2019-09-16

摘要

为了明确不同农杆菌菌种对茶多酚的耐受性能变化幅度,以及驯化培养对提高农杆菌茶多酚耐受性、从而提高茶树遗传转化中遗传转化率的作用与可行性,本文比较了茶多酚对四种农杆菌生长的抑制率,进行了耐酚菌株的驯化培养,最后比较了驯化菌株与原始菌株的转化能力。结果表明:茶多酚对几种农杆菌均有明显的生长抑制作用,但不同菌种的耐受性能差异较大;通过驯化培养得到的耐酚菌株EHA105AP,其耐酚性能提高了90.89%,同时,其在茶树叶片和愈伤中的GUS瞬时表达率也分别提高了43.85%和57.55%。

本文引用格式

仓梅芹 , 周健 , 成浩 , 黎星辉 , 王丽鸳 . 几种农杆菌的耐酚性能比较与驯化研究[J]. 茶叶科学, 2008 , 28(3) : 189 -194 . DOI: 10.13305/j.cnki.jts.2008.03.004

Abstract

In order to make clear the variation of polyphenol-resisting characteristics of different kinds of Agrobacterium as well as the potential in improving the polyphenol-resisting ability and the Agrobacterium-mediated genetic transformation rate by domestication culture, the inhibition effect of tea polyphenols on four kinds of Agrobacterium strains, the polyphenol-resisting demestication of Agrobacterium strains and the improvement of genetic transformation ability in demesticated polyphenol-resisting strain were studied. The results showed that tea polyphenols had obvious inhibition on the growth of different kinds of Agrobacterium, but it varied greatly among these studied Agrobacterium. A polyphenol-resisting strain EHA105AP was obtained by domestication culture procedure, the polyphenol-resisting ability of which enhanced 90.89% compared with that of the original. Meanwhile, the rate of transient expression of GUS gene induced by the domesticated strain increased 43.85% and 57.55% respectively in callus and leaf explants case.

参考文献

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