采用常规耗竭法和改进耗竭法研究了茶树氟的吸收动力学参数,对测定和数据处理方法进行了探讨。在常规耗竭法中,茶苗在0.1、0.2 mg/L和0.6 mg/L的起始氟浓度条件下生长,吸收84 h,每隔12 h取一次营养液,通过氟的浓度差来计算吸收量;在改进耗竭法中设置氟浓度梯度0.1、0.2、0.4、0.6、0.8、1 mg/L和2 mg/L,茶苗吸收24 h后,取营养液,通过氟的浓度差来计算吸收速率。结果表明,常规耗竭法和改进耗竭法、不同的数据处理方法所得的茶树氟吸收动力学参数值有较大差异。常规耗竭法中起始浓度对动力学参数产生了影响,因此其结果有不确定性;改良耗竭法是比较好的方法,用作图法和Sigma Plot分析的数据准确性较高,参数值较为合理。Vmax值分别为2.605(µg/g·h DW)、3.419(µg/g·h DW),Km分别为0.385 mg/L和0.541 mg/L。
Tea plant (Camellia sinensis L) was employed as a model plant to study fluoride (F) uptake kinetic using both the general depletion and modified depletion methods. General depletion was the method that put the tea into solution of a initial F concentration of 0.1 mg/L, 0.2 mg/L, 0.6 mg/L and sampled 2 ml of the solution every 12 h until 84 h. The absorptive quantity was calculated by the difference of F concentration. Modified depletion was the method that put the tea into solution of an initial F concentration of 0.1 mg/L, 0.2 mg/L, 0.4 mg/L, 0.6 mg/L, 0.8 mg/L, 1 mg/L, 2 mg/L and sampled 2 ml of the solution after 24 h. The absorptive rate was calculated by the difference of F concentration. The results on the kinetic parameter by using of the general depletion were different from those by using the modified depletion method. It is because of the initial F concentration. The modified depletion method was a preferable method and the parameters were much reasonable by using of the plotting method and Sigma Plot than the other method. Result showed that the method Vmax was 2.605(µg/g*h DW) and 3.419(µg/g*h DW), and Km was 0.385 mg/L and 0.541 mg/L respectively.
[1] 张乃明. 山西土壤氟含量分布及影响因素研究[J]. 土壤学报, 2001, 38(2): 284~287.
[2] 曾清如, 周细红, 毛小云. 氟化物对植物的衰老效应及其机理探讨[J]. 农业环境保护, 1996, 15(5): 231~233.
[3] Miller G W.The effect of fluoride on higher plants: with special emphasis on early physiological and biochemical disorders[J]. Fluoride, 1993(26): 3~22.
[4] Davison A W, Takmaz-Nisancioglu S, Bailey I F.The dynamics of fluoride accumulation by vegetation [A]. Susheela A K. Fluoride Toxicity[C]. New DolhI: International Society for Fluoride Research, 1985, 30~46.
[5] Xie ZM, Ye ZH, Wong MH.Distribution characteristics of fluoride and aluminium in soil profiles of an abandoned tea plantation and their uptake by six woody species[J]. Environment International, 2001, 26: 341~346.
[6] 田宵鸿, 李生秀, 王清君. 几种作物NO3-吸收动力学参数测定方法初探[J]. 土壤通报, 2001, 32(1): 16~18.
[7] 陆景陵. 植物营养学[M]. 北京: 中国农业大学出版社, 1994: 79~82.
[8] Kazunori, Tamai, Jian Feng Ma. Characterization of silicon uptake by rice roots[J]. New Phytologist, 2003(158): 431~436.
[9] 龙怀玉, 李韵珠. 双倒数法和优化法求算Michaelis-Menten方程参数的对比研究[J]. 中国农业大学学报, 1999, 4(6): 53~58.
[10] 郑达贤, 沙济琴. 福建茶区土壤中的氟[J]. 土壤通报, 1994, 25(5): 230~233.
[11] KF Fung, ZQ Zhang, JWC Wong, MH Wong.Fluoride Contents in Tea and Soil from Tea Plantations and the Release of Fluoride into Tea Liquor during Infusion[J]. Environmental Pollution, 1999(104): 197~205.
[12] 吴卫红, 谢正苗, 徐建明,等. 不同土壤中氟赋存形态特征及其影响因素[J]. 环境科学, 2002, 23(2): 104~108.
[13] 马立锋, 石元值, 阮建云, 等. 湘、鄂砖茶主产区茶园氟含量状况及影响因素[J]. 茶叶科学, 2002, 22(1): 34~37.
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