研究腊状芽孢杆菌(Bacillus megaterium)、K1菌株(胶质芽孢杆菌)(Bacillus mucilaginosus)及K2(胶质芽孢杆菌)、K3(胶质芽孢杆菌),两类细菌在实验室条件下的解磷、解钾作用,通过对特定波长、不同pH值条件下培养液的吸光度来确定单位体积菌数的多少,结果显示,在pH值为5.5~6.5之间的吸光度较高,即这些菌株能在此pH值范围内较好生长。将菌株发酵液与菜籽饼混合,配制成生物菌肥施入茶园土壤具有提高土壤速效养分含量、促进茶树新梢生长、增加茶叶产量的效果。结果表明:所选菌株作为菌种配制茶园生物菌肥,对提高土壤肥力,促进茶树生长,增加茶叶产量均有较好效果。
The phosphorus-releasing and potassium-releasing ability of two species of bacterium in soils of tea garden (pH5.5~6.5) was investigated under laboratory condition. The amounts of bacterium per unit volume were ascertained by the absorbance of culture solution under special wavelength and various pH. Results showed the absorbance was relatively high under pH5.5~6.5, that meant these bacterium grew well under the above pH range: the bacterial bio-fertilizer made of bacterial fermentation liquid and rapeseed cake can enhance the contents of fast-acting phosphorus and potassium, improve the growth of tea shoots and increase the tea yield after applied in the soils of tea garden. Results indicated that the selected bacterial strain and the produced bio-fertilizer showed good effectiveness in proving the soil fertility and growth of tea plant and increase the yield of tea garden.
[1] 吴洵. 茶园施肥的现状与展望(译)[J]. 土壤学进展. 1981, (3): 21~23.
[2] 韩文炎, 李强. 茶园施肥现状与无公害茶园高效施肥技术[J]. 中国茶叶, 2002, 24(6): 29~31.
[3] 刘建. 微生物肥料作用机理的初步研究[C]. 北京: 中国农业科学院硕士生论文, 2000.
[4] 郑传进, 黄林, 龚明. 巨大芽孢杆菌解磷能力的研究[J]. 江西农业大学学报(自然科学版), 2002, 24(2): 190~192.
[5] 盛下放. 硅酸盐细菌NBT菌株解钾机理初探[J]. 土壤学报, 土壤学报, 2002, 39(6): 31~36.
[6] 葛诚. 微生物肥料生产应用基础[M]. 北京: 中国农业科技出版社, 2000.
[7] 王平, 李阜棣. 几个有关细、真菌定殖的几个重要问题[J]. 土壤学进展, 1994, 22(6): 35~41.
[8] 王志成. 磷钾细菌的分离筛选与培养研究[J]. 长沙电力学院学报(自然科学版), 2002, 17(4): 76~78.
[9] 赵晨曦, 刘前刚, 张志元. 磷钾细菌解磷解钾能力研究[J]. 湖南农业大学学报(自然科学版), 2004, 30(6): 519~520.
[10] 万王洛, 康丽华, 廖宝文, 等. 红树林根际解磷菌分离,培养及解磷能力的研究[J]. 林业科学研究, 2004, 17(7): 89~94.
[11] 刘红艳, 张亚莲, 常硕其, 等. 茶园生物菌肥高效菌株的筛选[J]. 茶叶通讯, 2006, 23(4): 13~15.