研究了采用碱法从茶叶中提取非水溶性茶叶蛋白的工艺,茶叶蛋白提取率达61.1%。通过调节提取液pH值的方法制备粗蛋白,粗蛋白纯度为54.45%;以高血脂症大鼠为模型,研究了非水溶性茶叶蛋白的降血脂作用。结果表明:非水溶性茶叶蛋白能明显降低高血脂症大鼠血清TC、TG、LDL-C、AI和R-CHR指标(P<0.05),对HDL-C有一定的调节作用;证明非水溶性茶叶蛋白有明显的降血脂效果,对抗动脉粥样硬化及冠心病可能有一定的预防作用。
Alkali extraction technology of water insoluble protein from tea was studied. The extracting rate of tea protein was around 61.1%. The crude tea protein was prepared by adjusting pH of the extract and its purity was 54.45%.The effects of water insoluble tea protein on decreasing blood lipids were studied on the hyperlipidemia rats. Results showed that the protein had significant effects on decreasing TC、TG、LDL-C、AI and R-CHR in the hyperlipidemia rats(p<0.05). It could also modulate the HDL-C indexes. The results confirmed that the water insoluble tea protein had remarkable effects on decreasing the blood lipid of hyperlipidemia and possibly is preventing atherosclerosis and coronary heart disease.
[1] 安徽农学院. 茶叶生物化学, 第二版[M]. 北京, 农业出版社, 1988: 3~5.
[2] 汪东风, 谢晓凤, 严俊等. 茶多糖等有效成分综合提取研究[J]. 中草药, 1998, 29(11): 739~740.
[3] 李燕, 蔡东联, 等. 茶叶蛋白液预防辐射引起的突变效应[J]. 癌变·畸变·突变, 2001, 13(1): 32~35.
[4] 汪家政, 范明. 蛋白质技术手册[M]. 科学出版社, 2002: 65~67.
[5] 北京大学生物化学教研室. 生物化学实验指导[M]. 北京: 人民教育出版社, 1979.
[6] DOMINGUEZ H,NUNEZ M J.Enzymatic Pretreatment to Enhance Oil Extraction from Fruits and Oilseeds[J]. A Review Food Chemistry. 1994, 49: 291~286.
[7] ROBERT D HAGENMAIER.Aqueous Processing of Full-Fai Sunflower Seed[J]. Yields of Oil and Protein.1974: 470~471.
[8] 杨玺. 血脂异常及高粘度症防治必读[M]. 上海科学技术文献出版社, 2002: 18~30.
[9] 白堃元. 茶叶加工[M]. 北京,化学工业出版社, 2001: 1~6.
[10] 姚慧英, 刘启贵. 茶叶深加工的意义和作用[J]. 茶报, 2001(2): 30~32.