利用体外细胞培养技术探讨了四种儿茶素对ECV304细胞增殖和迁移的影响。结果发现，EGCG对ECV304细胞存活率的影响最显著，在EGCG浓度为50~150 μmol/L时，细胞存活率达到极显著差异，半数抑制浓度IC50为75~150 μmol/L；EGCG对该细胞迁移作用影响明显，且呈剂量依赖性，IC50为150 μmol/L，EGCG可能通过抑制内皮细胞的迁移抑制新生血管的生成；ECG与ECV304作用36 h和48 h，IC50分别为390 μmol/L和370 μmol/L；C和EC与ECV304作用时，细胞存活率在80%以上。四种儿茶素在浓度低于75 μmol/L时，对正常ECV304细胞抑制影响不大。
By employing techniques of cell culture, the effects of four tea catechins, EGCG, ECG, EC and C were investigated on the proliferation and migration of ECV304 by the authors. The result demonstrated that EGCG was the most effective in cell viability among the four tested catechins. Viability of ECV304 were significantly different in the case of the concentrations of EGCG ,which was ranged from 50 to 150 μmol/L. The IC50 of EGCG was 75~150 μmol/L, EGCG was also effective in cell migration in a dose dependent manner, the IC50 was 150 μmol/L. EGCG could inhibit angiogensis, which may be related to the decreased migration of endothelial cell. The IC50 was at 390 μmol/L and 370 μmol/L in 36 hour-disposal and 48 hour-disposal of ECG respectively. The viability of ECV304 disposed by C and EC were above 80%. It was indicated that catechins showed insignificant inhibitory effect on normal cell growth and migration at the concentration of lower than 75 μmol/L.
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