茯砖茶提取物对葡聚糖硫酸钠诱导小鼠炎症性肠病的保护作用研究

胡安恺; 姚立筠; 赵悦伶; 刘畅; 王岳飞; 徐平

doi:10.13305/j.cnki.jts.2019.06.003

茶叶科学 >

2019 , Vol. 39 >Issue 6: 641 - 651

DOI: https://doi.org/10.13305/j.cnki.jts.2019.06.003

茯砖茶提取物对葡聚糖硫酸钠诱导小鼠炎症性肠病的保护作用研究

胡安恺 ,
姚立筠 ,
赵悦伶 ,
刘畅 ,
王岳飞 ,
徐平

展开

1. 东北农业大学食品学院乳品工程系,黑龙江哈尔滨 150030;
2. 浙江大学茶叶研究所,浙江杭州 310058

胡安恺,男,本科,主要从事茶叶营养方面的研究。

收稿日期: 2019-07-19

修回日期: 2019-08-25

网络出版日期: 2019-12-24

收起

Protective Effects of Fu Brick Tea Extracts on Inflammatory Bowel Disease Induced by Dextran Sulfate Sodium in Mice

HU Ankai ,
YAO Liyun ,
ZHAO Yueling ,
LIU Chang ,
WANG Yuefei ,
XU Ping

Expand

1. Department of dairyengineering, Northeast Agricultural University, Haerbin 150030, China;
2. Tea Research Institute, Zhejiang Univerisity, Hangzhou 310058, China

Received date: 2019-07-19

Revised date: 2019-08-25

Online published: 2019-12-24

Fold

摘要

茯砖茶是我国特有的一种黑茶。本文以茯砖茶为原料,分别制备了茯砖茶水提物（FWE）、经95%乙醇沉后得到的醇溶物（FES）和醇沉物（FEP）,并探究了3种提取物对葡聚糖硫酸钠诱导小鼠炎症性肠病的保护作用。研究表明,3种茯砖茶提取物都能够减轻炎症性肠病的肠道结构损伤,其中FWE组效果最好;同时茯砖茶提取物可以显著提升小鼠血清和肠道组织上清液中超氧化物岐化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）水平以及降低白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）水平,且作用效果均为FWE组>FES组>FEP组;在蛋白水平上,FWE组小鼠肠道组织的NF-κB p65蛋白水平有所降低;IκBα的蛋白水平在FWE组和FES组中有所增加;核因子E2相关因子2（Nrf2）和血红素氧化酶-1（HO-1）的相关蛋白水平在FWE组和FES组也有所增加。综上所述,茯砖茶提取物可以通过抗氧化和抗炎途径对葡聚糖硫酸钠诱导小鼠炎症性肠病发挥预防保护作用。

关键词： 茯砖茶; 炎症性肠病（IBD）; 炎症; 氧化应激

本文引用格式

胡安恺 , 姚立筠 , 赵悦伶 , 刘畅 , 王岳飞 , 徐平 . 茯砖茶提取物对葡聚糖硫酸钠诱导小鼠炎症性肠病的保护作用研究[J]. 茶叶科学, 2019 , 39(6) : 641 -651 . DOI: 10.13305/j.cnki.jts.2019.06.003

Abstract

Fu brick tea is a special dark tea in China. In the present work, three extracts, including Fu brick tea water extract (FWE), Fu brick tea ethanol soluble fraction (FES) and Fu brick tea ethanol precipitation fraction(FEP), were prepared and used to further explore their protective effects on inflammatory bowel disease in mice. Researches show that all the Fu brick tea extracts could alleviate the intestinal structure damage of IBD mice. Moreover, the extracts increased the activities of SOD, CAT and the levels of GSH and reduced the levels of IL-6, IL-1β, TNF-α in serum and colon tissue supernatant of mice. Furthermore, the protein level of NF-κB p65 in the colon tissues in the FWE group was decreased compared to the model group. The protein level of IκBα was increased in the FWE group and FES group. Levels of Nrf2 and HO-1 were also increased in the FWE and FES groups. In conclusion, Fu brick tea extracts had a protective effect on IBD mice induced by DSS.

Key words： Fu brick tea; inflammatory bowel disease; inflammation; oxidative stress

参考文献

[1] Ng SC, Shi HY, Hamidi N, et al.Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies[J]. The Lancet, 2017, 390(10114): 2769-2778.
[2] Danese S, Fiocchi C.Ulcerative colitis[J]. New England Journal of Medicine, 2011, 365(18): 1713-1725.
[3] Ghosh S, Mitchell R.Impact of inflammatory bowel disease on quality of life: Results of the European Federation of Crohn's and Ulcerative Colitis Associations (EFCCA) patient survey[J]. Journal of Crohns & Colitis, 2007, 1(1): 10-20.
[4] Hashimoto K, Oshima T, Tomita T, et al.Oxidative stress induces gastric epithelial permeability through claudin-3[J]. Biochemical and Biophysical Research Communications, 2008, 376(1): 154-157.
[5] Neurath MF.Cytokines in inflammatory bowel disease[J]. Nature Reviews Immunology, 2014, 14(5): 329-342.
[6] Ng SC, Tang W, Leong RW, et al.Environmental risk factors in inflammatory bowel disease: a population-based case-control study in Asia-Pacific[J]. Gut, 2015, 64(7): 1063-1071.
[7] Wang YF, Qin OY, Xia B, et al.Multicenter case-control study of the risk factors for ulcerative colitis in China[J]. World Journal of Gastroenterology, 2013, 19(11): 1827-1833.
[8] Kim M, Murakami A, Miyamoto S, et al.The modifying effects of green tea polyphenols on acute colitis and inflammation-associated colon carcinogenesis in male ICR mice[J]. Biofactors, 2010, 36(1): 43-51.
[9] Bitzer ZT, Elias RJ, Matam VK, et al.(-)-Epigallocatechin-3-gallate decreases colonic inflammation and permeability in a mouse model of colitis, but reduces macronutrient digestion and exacerbates weight loss[J]. Molecular Nutrition & Food Research, 2016, 60(10): 2267-2274.
[10] Chen G, Xie M, Dai Z, et al.Kudingcha and fuzhuan brick tea prevent obesity and modulate gut microbiota in high-fat diet fed mice[J]. Molecular Nutrition & Food Research, 2018, 62(6): e1700485. DOI: 10.1002/mnfr.201700485.
[11] Chen G, Xie M, Wan P, et al.Fuzhuan brick tea polysaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with modulation in the gut microbiota[J]. Journal of Agricultural and Food Chemistry, 2018, 66(11): 2783-2795.
[12] Foster MT, Gentile CL, Kimberly CY, et al.Fuzhuan tea consumption imparts hepatoprotective effects and alters intestinal microbiota in high saturated fat diet-fed rats[J]. Molecular Nutrition & Food Research, 2016, 60(5): 1213-1220.
[13] Li Q, Liu Z, Huang J, et al.Anti-obesity and hypolipidemic effects of Fuzhuan brick tea water extract in high-fat diet-induced obese rats[J]. Journal of the Science of Food and Agriculture, 2013, 93(6): 1310-1316.
[14] Chen G, Wang M, Xie M, et al.Evaluation of chemical property, cytotoxicity and antioxidant activity in vitro and in vivo of polysaccharides from Fuzhuan brick teas[J]. International Journal of Biological Macromolecules, 2018, 116: 120-127.
[15] Liu Z, Lin Y, Zhang S, et al.Comparative proteomic analysis using 2DE-LC-MS/MS reveals the mechanism of Fuzhuan brick tea extract against hepatic fat accumulation in rats with nonalcoholic fatty liver disease[J]. Electrophoresis, 2015, 36(17): 2002-2016.
[16] 余智勇, 黄建安, 杨明臻, 等. 茯砖茶抗腹泻效果研究[J]. 茶叶科学, 2009, 29(6): 465-469.
[17] Liu B, Yang T, Zeng LN, et al.Crude extract of Fuzhuan brick tea ameliorates DSS-induced colitis in mice[J]. International Journal of Food Science and Technology, 2016, 51(12): 2574-2582.
[18] Xu YQ, Liu PP, Shi J, et al.Quality development and main chemical components of Tieguanyin oolong teas processed from different parts of fresh shoots[J]. Food Chemistry, 2018, 249: 176-183.
[19] 张勇. 铁皮石斛茎、叶、花多糖理化性质及抗氧化、免疫调节活性研究[D]. 杭州: 浙江大学, 2016.
[20] 何佳. E3泛素酶FBXW7正向调控小鼠炎症性肠病和CX3CR1(int)炎性巨噬细胞的聚集[D]. 杭州: 浙江大学, 2016.
[21] 张遴, 方悦. 茯茶功效研究进展[J]. 食品研究与开发, 2018, 39(9): 208-212.
[22] Boussenna A, Cholet J, GoncalvesMN, et al. Polyphenol-rich grape pomace extracts protect against dextran sulfate sodium-induced colitis in rats[J]. Journal of the Science of Food and Agriculture, 2016, 96(4): 1260-1268.
[23] Sabri S, Hsu YH, He JL, et al.Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice[J]. PLoS ONE, 2018, 13(10): e0205252. DOI: 10.1371/JOURNAL.PONE.0205252.
[24] Ramberg JE, Nelson ED, Jacoby HI, et al.Plant polysaccharide supplements reduce the expression of pro-inflammatory genes in colonic tissue of mice with dextran sulfate sodium-induced colitis[J]. International Journal of Probiotics and Prebiotics, 2010, 5(4): 229-236.
[25] Thiele K, Bierhaus A, Autschbach F, et al.Cell specific effects of glucocorticoid treatment on the NF-kappa Bp65/I kappa B alpha system in patients with Crohn's disease[J]. Gut, 1999, 45(5): 693-704.
[26] Atreya I, Atreya R, Neurath MF.NF-kappa B in inflammatory bowel disease[J]. Journal of Internal Medicine, 2008, 263(6): 591-596.
[27] Tak PP, Firestein GS.NF-kappa B: a key role in inflammatory diseases[J]. Journal of Clinical Investigation, 2001, 107(1): 7-11.
[28] Saber S, Khalil RM, Abdo WS, et al.Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NF-kappaB and Nrf-2/HO-1 signaling crosstalk[J]. Toxicol Appl Pharmacol, 2019, 364: 120-132.
[29] Akiyama S, Nesumi A, Mari MY, et al.Effects of anthocyanin-rich tea "Sunrouge" on dextran sodium sulfate-induced colitis in mice[J]. Biofactors, 2012, 38(3): 226-233.
[30] Li W, Khor TO, Xu C, et al.Activation of Nrf2-antioxidant signaling attenuates NF-kappaBinflammatory response and elicits apoptosis[J]. Biochem Pharmacol, 2008, 76(11): 1485-1489.
[31] Gerges GA, Rizzo M, Eid A, et al.Tea catechins induce crosstalk between signaling pathways and stabilize mast cells in ulcerative colitis[J]. Journal of Biological Regulators and Homeostatic Agents, 2017, 31(4): 865-877.
[32] Rahman SU, Li Y, Huang Y, et al.Treatment of inflammatory bowel disease via green tea polyphenols: possible application and protective approaches[J]. Inflammopharmacology, 2018, 26(2): 319-330.
[33] Carini F, Tomasello G, Jurjus A, et al.Colorectal cancer and inflammatory bowel diseases: effects of diet and antioxidants[J]. Journal of Biological Regulators and Homeostatic Agents, 2017, 31(3): 791-795.
[34] Grove KA, Sudathip ST, Kennett MJ, et al.(-)-Epigallocatechin-3-gallate inhibits pancreatic lipase and reduces body weight gain in high fat-fed obese mice[J]. Obesity, 2012, 20(11): 2311-2313.
[35] 王增盛, 施兆鹏, 刘仲华, 等. 论茯砖茶品质风味形成机理[J]. 茶叶科学, 1991(S1): 49-55.
[36] 刘玉倩, 杨家干. 茯砖茶发花过程菌落及主要内含物质变化[J]. 蚕桑茶叶通讯, 2017(5): 30-33.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献