PBD和RSM联用优化聚酯型儿茶素A化学合成技术参数

张建勇; 陈琳; 崔宏春; 王伟伟; 薛金金; 熊春华; 江和源

doi:10.13305/j.cnki.jts.20200117.002

茶叶科学 >

2020 , Vol. 40 >Issue 1: 51 - 62

DOI: https://doi.org/10.13305/j.cnki.jts.20200117.002

PBD和RSM联用优化聚酯型儿茶素A化学合成技术参数

张建勇 ,
陈琳 ,
崔宏春 ,
王伟伟 ,
薛金金 ,
熊春华 ,
江和源

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1. 浙江工商大学食品与生物工程学院,浙江杭州 310018;
2. 中国农业科学院茶叶研究所,浙江杭州 310008;
3. 杭州市农业科学研究院茶叶研究所,浙江杭州 310024

张建勇，男，助理研究员，博士在读，主要从事茶叶化学与加工方面的研究。

收稿日期: 2019-11-04

修回日期: 2019-12-22

网络出版日期: 2020-02-04

基金资助

国家自然科学基金（31670692）、浙江省自然科学基金（LY18C160004）、公益性行业专项（201503142-11）、国家茶叶产业技术体系（CARS-23-03A）

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Optimization of Technical Parameters for Chemical Synthesis of Theasinensin A by PBD and RSM

ZHANG Jianyong ,
CHEN Lin ,
CUI Hongchun ,
WANG Weiwei ,
XUE Jinjin ,
XIONG Chunhua ,
JIANG Heyuan

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1. School of Food Science and biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China;
2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Tea Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received date: 2019-11-04

Revised date: 2019-12-22

Online published: 2020-02-04

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摘要

为探明化学合成条件对聚酯型儿茶素A（Theasinensin A,TSA）得率的影响,通过单因素试验和Plackett-Burman Design（PBD）确定TSA化学合成关键因子,然后采用响应面法（Response surface methodology,RSM）,进一步优化TSA化学合成技术参数。结果表明,氯化铜用量、甲醇体积分数、温度对TSA得率的影响差异极显著,主因素效应为甲醇体积分数>氯化铜用量>温度,最优条件为氯化铜用量43%,甲醇体积分数26%,温度15℃,聚酯型儿茶素得率为59.12%,与模型预测值59.34%接近。PBD和RSM联用优化TSA化学合成工艺可行,预测性较好,可为其他种类儿茶素氧化聚合物的高效化学合成提供借鉴和理论依据。

关键词： TSA; EGCG; 化学合成; PBD; RSM; 优化

本文引用格式

张建勇 , 陈琳 , 崔宏春 , 王伟伟 , 薛金金 , 熊春华 , 江和源 . PBD和RSM联用优化聚酯型儿茶素A化学合成技术参数[J]. 茶叶科学, 2020 , 40(1) : 51 -62 . DOI: 10.13305/j.cnki.jts.20200117.002

Abstract

The effect of chemical synthesis conditions on the yield of theasinensin A was studied. Key factors for chemical synthesis of theasinensin A (TSA) were determined by single factor test and Plackett-Burman Design (PBD). The response surface methodology (RSM) was employed to optimize the technical parameters for the chemical synthesis. The experiment results indicate that the effects of copper oxide content, methanol content and temperature on the yield were extremely significant. The main factors were methanol content, next copper oxide content, and then temperature. The optimal conditions were as follows: copper chloride content 43%, methanol content 26%, and temperature 15℃. Under these conditions, the yield of TSA was 59.12%, which was close to the predicted value of 59.34%. The combination of PBD and RSM to optimize the chemical synthesis of TSA achieved ideal result, which could provide reference and theoretical basis for the efficient chemical synthesis of other catechin oxidation polymers.

Key words： theasinensin A (TSA); EGCG; chemical synthesis; PBD; RSM; optimization

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