硅肺是吸入SiO2粉尘引起的以肺间质纤维化为主的全身性疾病。探讨了白茶提取物对纳米SiO2诱导大鼠肺纤维化的抑制作用及机制。54只Wistar大鼠随机分为正常组、模型组、白毫银针提取物组、白牡丹提取物高和低剂量组、EGCG组共6个组,每组9只大鼠。除正常组外的其余5个组采用非暴露式气管插管方法造模纳米SiO2粉尘(80 mg·mL-1),每天以灌胃方式给予药物两周之后,检测肺组织中羟脯氨酸(HYP)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)和谷光甘肽过氧化物酶(GSH-Px)含量以及肺组织形态学变化。结果显示,与模型组相比,白茶提取物各处理组及EGCG组病理形态改变有不同程度的减轻,其中白毫银针提取物组的效果最佳。白茶提取物各处理组与EGCG组的大鼠肺NO含量和炎症因子IL-6都显著低于模型组(P<0.05),GSH-Px活力高于模型组(P<0.05),高剂量白牡丹提取物对降低NO含量和升高GSH-Px活力效果最好;本研究表明,白茶提取物对于纳米SiO2引起的大鼠肺纤维化氧化应激损伤具有有效的减缓和修复作用,主要与其抗氧化作用和抑制炎症反应相关。
Fifty-four Wistar rats were randomly divided into six groups: control group, model group, white silver needle extract group, high and low dose white peony extracts and EGCG group, with 9 rats in each group. The other five groups except the control group were treated with nano-sized SiO2 dust (80 mg·mL-1) by non-exposed endotracheal intubation. After two weeks of intragastric administration, the contents of hydroxyproline(HYP), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and the morphological changes of lung tissues were detected. The results show that compared with the model group, the pathological changes of each white tea extract treatment group and EGCG group were alleviated in varying degrees, and the effect of white silver needle extract group was the best. The content of NO and inflammatory factor IL-6 in lung of rats treated with white tea extract and EGCG were significantly lower than those of model group (P<0.05), GSH-Px activity was higher than that of model group (P<0.05). High-dose white peony extract group had the best effect on reducing NO content and increasing GSH-Px activity. This study shows that white tea extract had a significant effect on oxidative stress injury of lung fibrosis induced by nano-sized SiO2 in rats. The slow and repairing effects are mainly related to the antioxidant effect and the inhibition of inflammatory reaction.
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