不同品种茶树根际AM真菌群落结构分析

何斐; 李冬花; 卜凡

doi:10.13305/j.cnki.jts.2020.03.003

茶叶科学 >

2020 , Vol. 40 >Issue 3: 319 - 327

DOI: https://doi.org/10.13305/j.cnki.jts.2020.03.003

不同品种茶树根际AM真菌群落结构分析

何斐 ,
李冬花 ,
卜凡

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安康学院现代农业与生物科技学院,陕西安康 725000

何斐,女,副教授,主要从事微生物资源利用方面的研究。hefei6000@163.com

收稿日期: 2019-10-27

修回日期: 2019-12-24

网络出版日期: 2020-06-09

基金资助

陕西省科协青年人才托举计划项目（20170210）、陕西省教育厅自然科学专项研究计划项目（17JK0016）、安康学院高层次人才科研启动项目（2017AYQDZR06）、国家级大学生创新创业训练计划项目（201711397007、201711397006）

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Analysis of Arbuscular Mycorrhizal Fungal Community Structure in the Rhizosphere of Different Tea Cultivars

HE Fei ,
LI Donghua ,
BU Fan

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School of Agriculture & Biotechnology, Ankang University, Ankang 725000, China

Received date: 2019-10-27

Revised date: 2019-12-24

Online published: 2020-06-09

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摘要

对陕西安康汉水韵茶园栽培的5个品种茶树根际丛枝菌根（Arbuscular mycorrhiza,AM）真菌群落结构特征进行分析,以期丰富我国茶树AM真菌种质资源库。结果表明,不同品种茶树根际AM真菌种丰度及种属组成等存在差异。其中,紫阳群体种茶树根际分离的AM真菌最多（6种）,陕茶1号、龙井长叶、龙井43和福鼎大白茶各分离到5、4、4种和3种。龙井长叶茶树根际AM真菌孢子密度最高（每克干土含3.57个孢子）,龙井43最低（每克干土含1.10个孢子）。紫阳群体种茶树的AM真菌物种多样性Shannon-Wiener和均匀度指数均达到最高,分别为0.63和0.096,龙井长叶最低（0.18和0.027）。龙井长叶的菌根定殖率最高（29.5%）,福鼎大白最低（15.8%）。不同茶树品种AM真菌种类组成的相似性系数维持在0.111~0.750,其中,龙井长叶与龙井43茶树根际AM真菌种类组成相似性系数最高,而福鼎大白和紫阳群体种相似性系数最低。研究表明,不同品种茶树根际AM真菌群落结构存在一定的差异,根际土壤中鉴定的AM真菌资源对进一步筛选和研发茶树专用AM真菌菌剂,促进茶产业发展具有重要意义。

关键词： AM真菌; 茶树; 品种; 群落结构

本文引用格式

何斐 , 李冬花 , 卜凡 . 不同品种茶树根际AM真菌群落结构分析[J]. 茶叶科学, 2020 , 40(3) : 319 -327 . DOI: 10.13305/j.cnki.jts.2020.03.003

Abstract

In order to enrich the arbuscular mycorrhizal (AM) fungal germplasm resources of tea plants (Camellia sinensis) in China, the community structure of AM fungi in the rhizosphere soil of different tea cultivars grown in Hanshuiyun tea garden of Ankang City, Shaanxi Province were analyzed. The results show that species richness, species and genera composition of AM fungi in the rhizosphere soil varied with tea cultivars. A total of six AM fungal species were isolated from the rhizosphere soil of Ziyang population. Likewise, five from Shancha 1, four from Longjing Changye, four from Longjing 43, and three species from Fuding Dabai. Soil collected from the rhizosphere of Longjing Changye had the highest spore density (3.57 spores per gram of dry soil), while the lowest spore density (1.10 spores per gram of dry soil) was found in the rhizosphere of Longjing 43. The highest Shannon-Wiener and Pielou evenness indices were found in the rhizosphere of Ziyang population (0.63 and 0.096), whereas the lowest values were observed in the rhizosphere of Longjing Changye (0.18 and 0.027). The maximum mycorrhizal colonization (29.5%) was found in the rhizosphere of Longjing Changye, whereas the minimum value (15.8%) was observed in the rhizosphere of Fuding Dabai. The Sorenson’s similarity coefficient of AM fungal species composition among five tested tea cultivars ranged from 0.111 to 0.750, with the highest between Longjing Changye and Longjing 43, and the lowest between Fuding Dabai and Ziyang population. The results reveal obvious differences in AM fungal community composition among the five tea cultivars. The identified AM fungal resources in rhizosphere soil are of great significance for further screening, researching AM fungi agent, and promoting the development of tea industrialization.

Key words： AM fungi; Camellia sinensis; cultivar; community structure

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