3种抗生素处理对灰茶尺蛾内生菌群的影响

王志博; 白家赫; 周孝贵; 郭华伟; 肖强

doi:10.13305/j.cnki.jts.20201130.001

茶叶科学 >

2021 , Vol. 41 >Issue 1: 90 - 100

DOI: https://doi.org/10.13305/j.cnki.jts.20201130.001

研究报告

3种抗生素处理对灰茶尺蛾内生菌群的影响

王志博 ,
白家赫 ,
周孝贵 ,
郭华伟 ,
肖强

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中国农业科学院茶叶研究所,农业农村部茶叶质量安全控制重点实验室,浙江杭州310008

王志博,男,副研究员,主要从事茶园病虫害防治研究,wangzhibo9527@163.com。

收稿日期: 2020-08-12

修回日期: 2020-09-30

网络出版日期: 2021-02-23

基金资助

国家自然科学基金（31700613）、中国农业科学院创新工程（CAAS-ASTIP-TRICAAS）

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Effect of Three Antibiotic Treatments on Bacterial Endosymbiont Community of Ectropis Grisescens Warren

WANG Zhibo ,
BAI Jiahe ,
ZHOU Xiaogui ,
GUO Huawei ,
XIAO Qiang

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Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received date: 2020-08-12

Revised date: 2020-09-30

Online published: 2021-02-23

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摘要

为明确不同抗生素对灰茶尺蛾内共生菌群落的影响,分别选用质量浓度2.5 mg·mL^-1的四环素、头孢氨苄、利福平3种抗生素溶液浸泡新鲜茶树叶片持续饲喂灰茶尺蛾幼虫3代,通过16 S rDNA高通量测序技术对内生菌群群落变化展开研究,并进一步基于wsp基因分子标记对灰茶尺蛾沃尔巴克氏体菌去除效果进行检测。结果表明,3种抗生素处理后,灰茶尺蛾菌群落均表现为丰富度增加、多样性减小;四环素和利福平饲喂处理能够成功将灰茶尺蛾沃尔巴克氏体菌去除,进而建立了不携带沃尔巴克氏体菌的灰茶尺蛾种群,为进一步开展沃尔巴克氏体菌的功能研究奠定了基础。

关键词： 灰茶尺蛾; 共生菌; 沃尔巴克氏体; 抗生素

本文引用格式

王志博 , 白家赫 , 周孝贵 , 郭华伟 , 肖强 . 3种抗生素处理对灰茶尺蛾内生菌群的影响[J]. 茶叶科学, 2021 , 41(1) : 90 -100 . DOI: 10.13305/j.cnki.jts.20201130.001

Abstract

In order to study the effects of three antibiotics on bacterial endosymbiont community of Ectropis grisescens, relative experiment was carried out with three antibiotics (tetracycline, cefalexin and rifampicin) at the concentration of 2.5 mg·mL^-1. Larvae of E. grisescens were fed the tea leaves with antibiotics for continuous 3 generations. Variation of endosymbiont community was studied using 16 S rDNA Illumina MiSeq technique. Besides, the Wolbachia of E. grisescens were detected basing on wsp gene. The results show that abundance and diversity of endosymbiont community in E. grisescens were increased and reduced, respectively under antibiotic treatments. The tetracycline and rifampicin could effectively remove Wolbachia from E. grisescens. Further, the population of E. grisescens without Wolbachia was established, which laid the foundation for the study on function of Wolbachia.

Key words： Ectropis grisescens; symbiotic bacteria; Wolbachia; antibiotics

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