茶树PPR基因家族全基因组鉴定及白化相关基因表达分析

刘丁丁; 王君雅; 汤榕津; 陈亮; 马春雷

doi:10.13305/j.cnki.jts.2021.02.002

茶叶科学 >

2021 , Vol. 41 >Issue 2: 159 - 172

DOI: https://doi.org/10.13305/j.cnki.jts.2021.02.002

研究报告

茶树PPR基因家族全基因组鉴定及白化相关基因表达分析

刘丁丁 ,
王君雅 ,
汤榕津 ,
陈亮 ,
马春雷

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1.中国农业科学院茶叶研究所/国家茶树改良中心/农业部茶树生物学与资源利用重点实验室,浙江杭州 310008;
2.中国农业科学院研究生院,北京 100081

刘丁丁,女,硕士研究生,主要从事茶树资源育种与遗传改良研究。

收稿日期: 2020-07-13

修回日期: 2020-09-15

网络出版日期: 2021-04-13

基金资助

中国农业科学院科技创新工程（CAAS-ASTIP-2017-TRICAAS）、现代农业产业技术体系（CARS-19）、浙江省农业（茶树）新品种选育重大科技专项子课题（2016C02053-5）、中国农业科学院茶叶研究所基本科研业务费（1610212017008、1610212019004）

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Genome-wide Identification of PPR Gene Family and Expression Analysis of Albino Related Genes in Tea Plants

LIU Dingding ,
WANG Junya ,
TANG Rongjin ,
CHEN Liang ,
MA Chunlei

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1. Tea Research Institute of the Chinese of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Hangzhou 310008, China;
2. Graduate School of Chinese Academy of Agriculture Science, Beijing 100081, China

Received date: 2020-07-13

Revised date: 2020-09-15

Online published: 2021-04-13

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摘要

三角状五肽（PPR）蛋白作为一类靶向定位于半自主细胞器的序列特异性RNA结合蛋白,在植物的生长发育过程中具有重要的作用。本研究基于茶树基因组数据,利用生物信息学方法对茶树CsPPR基因家族进行系统鉴定,并对其蛋白质理化性质、结构域保守性、亚细胞定位、基因结构、染色体定位与分布等进行了分析。结果表明,在茶树基因组数据中共鉴定到858个CsPPR基因,分属于P和PLS两个亚家族;结构域分析表明各个结构域在茶树中比较保守;亚细胞定位结果显示超过一半的CsPPR蛋白定位于叶绿体;基因结构分析表明茶树CsPPR基因家族中共有31%的CsPPR基因不具有内含子结构,并且家族成员在进化过程中发生过多次基因复制事件。另外,为探究茶树CsPPR基因家族在调控茶树白化相关基因表达过程中的作用,对正常叶色品种舒茶早和安吉黄茶等5个白化茶树品种进行转录组测序,共筛选到24个差异共表达CsPPR基因,并利用实时荧光定量PCR技术对其在不同品种和不同组织中的表达模式进行了分析,结果显示大多数CsPPR基因在新梢、成熟叶和茎中高表达,但在花和根中痕量表达。本研究结果可为茶树CsPPR家族成员的基因克隆和功能研究提供参考。

关键词： 茶树; PPR基因家族; 转录组; 生物信息学; 实时荧光定量PCR

本文引用格式

刘丁丁 , 王君雅 , 汤榕津 , 陈亮 , 马春雷 . 茶树PPR基因家族全基因组鉴定及白化相关基因表达分析[J]. 茶叶科学, 2021 , 41(2) : 159 -172 . DOI: 10.13305/j.cnki.jts.2021.02.002

Abstract

Pentatricopeptide repeat (PPR) proteins are a kind of sequence-specific RNA binding proteins and targeted at semi-autonomous organelles, which play essential roles in play growth and development. In this study, the CsPPR genes were systematically identified by bioinformatics analysis based on the tea genome data. Then, their subcellular localization, physicochemical properties, gene structures, the chromosome locations and distribution were analyzed. The results show that a total of 858 putative CsPPR members were obtained from the genome data, which belong to P and PLS subfamilies. Domain analysis shows that each domains were relatively conservative in tea plants. Subcellular localization prediction indicates more than half of CsPPR proteins were located in the chloroplasts. Gene structure analysis shows that 31% of CsPPR genes lacked intron and the gene family had undergone extensive gene duplication events in the process of evolution. Subsequently, In order to investigate the role of CsPPR gene family in regulating the gene expressions of albino tea plants, transcriptome analysis was performed on the normal leaf color cultivar ‘Shuchazao’ and five albino tea cultivars such as ‘Anji Huangcha’. And 24 differential co-expressed CsPPR genes were identified from transcriptome data of five groups, and the real-time quantitative PCR technology was used to analysis the expression pattern of the 24 CsPPR genes in different cultivars and tissues of tea plants, and the results show that the majority of them were highly expressed in shoots, mature leaves and stems. The research results would provide a basis for CsPPR gene cloning and functional research.

Key words： tea plants; PPR gene family; transcriptome; bioinformatics; real-time quantitative PCR

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