化学感受蛋白直系同源基因CSP8在茶尺蠖及近缘种灰茶尺蠖中的表达分析

严玉婷; 李玉杰; 王倩; 唐美君; 郭华伟; 李红亮; 孙亮

doi:10.13305/j.cnki.jts.2022.02.007

茶叶科学 >

2022 , Vol. 42 >Issue 2: 200 - 210

DOI: https://doi.org/10.13305/j.cnki.jts.2022.02.007

研究报告

化学感受蛋白直系同源基因CSP8在茶尺蠖及近缘种灰茶尺蠖中的表达分析

严玉婷 ,
李玉杰 ,
王倩 ,
唐美君 ,
郭华伟 ,
李红亮 ,
孙亮

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1.中国计量大学生命科学学院/浙江省生物计量及检验检疫技术重点实验室,浙江杭州 310018;
2.中国农业科学院茶叶研究所/农业农村部茶叶质量安全控制实验室,浙江杭州 310008;
3.河南科技学院植物保护系,河南新乡 453003;
4.浙江农林大学农业与食品科学学院,浙江杭州 311300

严玉婷,女,硕士研究生,主要从事昆虫生化与分子生物学方面的研究。

收稿日期: 2021-03-19

修回日期: 2021-04-14

网络出版日期: 2022-04-15

基金资助

中国科协青年人才托举工程项目（2018QNRC001）、中央级公益性科研院所基本科研业务费专项(1610212020001,1610212018010)、国家自然科学基金（31501652,31871977）

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The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren

YAN Yuting ,
LI Yujie ,
WANG Qian ,
TANG Meijun ,
GUO Huawei ,
LI Hongliang ,
SUN Liang

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1. College of Life Science, China Jiliang University/Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, Hangzhou 310018, China;
2. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang 453003, China;
4. College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou 311300, China

Received date: 2021-03-19

Revised date: 2021-04-14

Online published: 2022-04-15

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摘要

化学感受蛋白（Chemosensory proteins,CSPs）在昆虫化学通讯及其他生理过程中发挥重要功能。茶尺蠖与其近缘种灰茶尺蠖是茶园重要鳞翅目害虫,对我国茶叶的安全生产造成严重危害。研究化学感受蛋白直系同源基因在茶尺蠖两近缘种间的表达,有助于研发同时针对该两种害虫的绿色防控技术。通过荧光定量qRT-PCR分析了EoblCSP8直系同源基因EgriCSP8在灰茶尺蠖中的表达分布。结果表明,EgriCSP8与EoblCSP8具有保守的幼虫期高表达模式,EgriCSP8主要表达于灰茶尺蠖3龄幼虫头部。进一步比较Eobl-EgriCSP8在茶尺蠖两近缘种间的表达发现,EoblCSP8在茶尺蠖中的表达量显著高于EgriCSP8在灰茶尺蠖中的表达量。此外,还分析了沃尔巴克氏体菌（Wolbachia）及挥发物诱导对Eobl-EgriCSP8表达的影响,发现Wolbachia显著影响EgriCSP8在灰茶尺蠖中的表达。该结果证实Wolbachia参与调控昆虫CSPs基因的表达,为后续揭示Wolbachia介导CSPs表达,参与调控茶尺蠖两近缘种化学感受及与茶树的协同进化提供了新的研究思路。

关键词： 茶尺蠖; 灰茶尺蠖; 化学感受蛋白; Wolbachia; 表达分析

本文引用格式

严玉婷 , 李玉杰 , 王倩 , 唐美君 , 郭华伟 , 李红亮 , 孙亮 . 化学感受蛋白直系同源基因CSP8在茶尺蠖及近缘种灰茶尺蠖中的表达分析[J]. 茶叶科学, 2022 , 42(2) : 200 -210 . DOI: 10.13305/j.cnki.jts.2022.02.007

Abstract

Chemosensory proteins (CSPs) play crucial roles in insect chemosensory and non-chemosensory processes. Ectropis obliqua Prout and its sibling species, Ectropis grisescens Warren are serious lepidopteran moth pests in tea gardens and they cause destructive damages to tea plants. The development of greener and environmentally friendly pest managements would benefit from orthologous CSPs’ investigation on the interaction between two Ectropis species and tea plants. This study mainly examined the expression profiles of EgriCSP8, the orthologs of EoblCSP8 in E. grisescens by using the real-time quantitative PCR experiment. The qRT-PCR results show that EgriCSP8 and EoblCSP8 had a conserved larvae-enriched expression pattern. EgriCSP8 was mainly expressed in the heads of the third instar of E. grisescens larvae. Next, the expression levels of CSP8 were compared between E. obliqua and E. grisescens. The results show that the expression level of EoblCSP8 in E. obliqua was higher than that of EgriCSP8 in E. grisescens. In addition, the effects of Wolbachia and tea plant volatiles on CSP8 expression levels in E. obliqua and E. grisescens were also estimated. The results show that the EgriCSP8 expression level declined significantly when Wolbachia was removed in E. grisescens, which provided the first evidence that the insect CSPs’ expression is associated with Wolbachia. These findings laid a foundation for the future studies on the molecular mechanisms of chemosensory and coevolution between the geometrid sibling species and tea plants.

Key words： Ectropis obliqua Prout; Ectropis grisescens Warren; chemosensory protein; Wolbachia; expression analysis

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