茶树叶绿体基因组SNP分子标记的初步研究

罗祥宗; 胡云飞; 吴淋慧; 赵雅琦; 郑伟铭; 黎巷汝; 李力

doi:10.13305/j.cnki.jts.2022.06.012

茶叶科学 >

2022 , Vol. 42 >Issue 6: 768 - 778

DOI: https://doi.org/10.13305/j.cnki.jts.2022.06.012

研究报告

茶树叶绿体基因组SNP分子标记的初步研究

罗祥宗 ,
胡云飞 ,
吴淋慧 ,
赵雅琦 ,
郑伟铭 ,
黎巷汝 ,
李力

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1.武夷学院茶与食品学院,福建南平 354300;
2.安徽农业大学茶与食品科技学院,安徽合肥 230036;
3.福建农林大学园艺学院,福建福州 350002

罗祥宗,男,硕士研究生,主要从事茶树抗逆栽培及育种研究,1950888454@qq.com。

收稿日期: 2022-09-09

修回日期: 2022-11-01

网络出版日期: 2023-01-04

基金资助

福建省自然科学基金项目（2021J011135）

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Preliminary Study on SNP Molecular Markers in Tea Chloroplast Genome

LUO Xiangzong ,
HU Yunfei ,
WU Linhui ,
ZHAO Yaqi ,
ZHENG Weiming ,
LI Xiangru ,
LI Li

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1. Wuyi University College of Tea and Food, Nanping 354300, China;
2. Anhui Agricultural University College of Tea and Food Technology, Hefei 230036, China;
3. Fujian Agriculture and Forestry University, College of Horticulture, Fuzhou 350002, China

Received date: 2022-09-09

Revised date: 2022-11-01

Online published: 2023-01-04

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摘要

传统的叶绿体基因分子标记在茶组植物分类系统研究中的应用价值相对有限,为了筛选可用于茶树鉴别与母系溯源的SNP（单核苷酸多态性）位点组合,将18个已报道的茶组植物叶绿体全基因组序列进行比对,通过设计通用引物,在169个茶树品种/品系中进行候选分子标记扩增与一代测序分析,筛选出16对引物,共含25个SNP位点可用于茶树品种母系溯源与鉴别分析。另外,将SNP位点组成的DNA指纹图谱结合茶树品种资源基本信息进行数字编码,最终形成由30位数字组成的茶树品种资源分子身份证,并构建相应的条形码和二维码用于品种识别。本研究数据为茶树品种的母本溯源与鉴别提供新的思路。

关键词： 叶绿体基因; SNP分子标记; 母系溯源; 鉴别; 身份证

本文引用格式

罗祥宗 , 胡云飞 , 吴淋慧 , 赵雅琦 , 郑伟铭 , 黎巷汝 , 李力 . 茶树叶绿体基因组SNP分子标记的初步研究[J]. 茶叶科学, 2022 , 42(6) : 768 -778 . DOI: 10.13305/j.cnki.jts.2022.06.012

Abstract

The application value of traditional chloroplast gene molecular markers in tea section classification is limited relatively. The aim of this study was to screen out SNP (single nucleotide polymorphism) combinations that could be used for tea plant identification and maternal traceability. In this study, the chloroplast genome sequences of 18 reported tea plants were compared. By designing universal primers, the candidate molecular markers were amplified and analyzed by sanger sequencing in 169 tea cultivars/strains, and 16 pairs of primers containing 25 SNP loci were screened out. This set of SNP molecular markers can be used for maternal traceability and identification analysis of tea cultivars. In addition, the DNA fingerprints composed of SNP sites were digitally encoded in combination with the basic information of tea resources, and finally a 30-digit molecular ID card of tea resources was formed, and corresponding barcodes and two-dimensional codes were constructed for cultivar identification. The data from this study provided a new idea for maternal origin tracing and identification of tea cultivars.

Key words： chloroplast gene; SNP molecular markers; maternal traceability; identification; ID card

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