不同茶树品种叶片细胞壁氟富集差异探究

刘昱; 杨培迪; 张培凯; 詹文礼; 李游; 姚苏航; 赵洋; 成杨; 刘振; 沈程文

doi:10.13305/j.cnki.jts.2024.05.003

茶叶科学 >

2024 , Vol. 44 >Issue 5: 735 - 746

DOI: https://doi.org/10.13305/j.cnki.jts.2024.05.003

研究报告

不同茶树品种叶片细胞壁氟富集差异探究

刘昱 ,
杨培迪 ,
张培凯 ,
詹文礼 ,
李游 ,
姚苏航 ,
赵洋 ,
成杨 ,
刘振 ,
沈程文

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1.湖南农业大学茶学教育部重点实验室,湖南长沙 410128;
2.湖南省茶叶研究所,湖南长沙 410125

刘昱,男,硕士研究生,主要从事茶树栽培育种方面的研究。

收稿日期: 2024-07-19

修回日期: 2024-09-02

网络出版日期: 2024-11-08

基金资助

湖南省农业科技创新资金项目（2023CX68）、国家现代农业产业技术体系（CARS-19）、国家重点研发计划（2022YFD1600801）

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Investigation of Differential Fluorine Enrichment in Leaf Cell Walls of Different Tea Cultivars

LIU Yu ,
YANG Peidi ,
ZHANG Peikai ,
ZHAN Wenli ,
LI You ,
YAO Suhang ,
ZHAO Yang ,
CHENG Yang ,
LIU Zhen ,
SHEN Chengwen

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1. Key Lab of Education Ministry of Hunan Agricultural University for Tea Science, Changsha 410128, China;
2. Tea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China

Received date: 2024-07-19

Revised date: 2024-09-02

Online published: 2024-11-08

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摘要

茶树是一种氟超富集植物,不同茶树品种的叶片富集氟含量存在差异。为探究茶树叶片细胞壁氟富集规律,检测了15个茶树品种的叶片氟含量,以及叶片果胶、半纤维素、纤维素与其对应的氟含量,分析不同成熟度叶片细胞壁氟分布。结果显示,果胶是体现品种细胞壁氟富集成熟度差异的关键细胞壁组分。15个品种中,涟茶7号、槠叶齐、上梅洲种的成熟叶果胶含量与果胶氟含量都比嫩叶高,而铁观音是唯一成熟叶比嫩叶中果胶氟含量低的品种。细胞壁组分氟占比的相关性分析表明,茶树叶片成熟度越高,氟含量越高,且均呈细胞壁多个组分富集氟的趋势。通过综合评价及聚类分析筛选,茶树品种寒绿成熟叶组和嫩叶组均具有高细胞壁氟富集水平（D_1-4=0.704 6,D_5-8=0.928 6）;福鼎大白茶、湘波绿、湘波绿2号、碧云、黔湄702具有中等细胞壁氟富集水平（D_1-4=0.326 7~0.486 1,D_5-8=0.484 4~0.699 3）;云南乌黑大叶、槠叶齐具有低细胞壁氟富集水平（D_1-4=0.146 5~0.268 8,D_5-8=0.222 3~0.345 7）。本研究从品种和叶片成熟度角度探究茶树细胞壁氟富集规律及其差异性,为低氟茶树分子育种提供品种参考。

关键词： 茶树品种; 氟; 叶片细胞壁; 成熟度

本文引用格式

刘昱 , 杨培迪 , 张培凯 , 詹文礼 , 李游 , 姚苏航 , 赵洋 , 成杨 , 刘振 , 沈程文 . 不同茶树品种叶片细胞壁氟富集差异探究[J]. 茶叶科学, 2024 , 44(5) : 735 -746 . DOI: 10.13305/j.cnki.jts.2024.05.003

Abstract

Tea plants are known to hyperaccumulate fluorine, with significant variation in fluorine accumulation among different cultivars. To explore the patterns of fluorine accumulation in tea leaf cell walls, the fluorine content in leaves of 15 tea cultivars was measured. Moreover, the contents of pectin, hemicellulose, cellulose components, and their respective fluorine contents were analyzed. The distribution of fluorine in the cell walls at different maturity stages of leaves were examined. The results indicate that pectin is a crucial component reflecting the differences in fluoride accumulation and maturity level of the cell walls among cultivars. Only the mature leaves of ‘Liancha 7', ‘Zhuyeqi' and ‘Shangmeizhouzhong' increased pectin content and fluoride content in pectin compared to the young leaves. ‘Tieguanyin' was the only cultivar with the fluoride content in pectin decreased in the mature leaves compared to the young leaves. Correlation analysis of the fluoride proportion in the cell wall components reveals that higher fluoride content in the leaves correlates with greater maturity, reflecting a trend of multi-component fluoride accumulation in the cell walls. Through a comprehensive evaluation and cluster analysis, ‘Hanlü' exhibits consistently high levels of fluorine accumulation in cell walls at different maturity stages (D_1-4=0.704 6, D_5-8=0.928 6). ‘Fuding Dabaicha', ‘Xiangbolü', ‘Xiangbolü 2', ‘Biyun', and ‘Qianmei 702' show moderate levels of fluorine enrichment in cell walls (D_1-4=0.3267-0.4861, D_5-8=0.484 4~0.699 3), while ‘Yunnan Wuheidaye' and ‘Zhuyeqi' exhibit low levels of fluorine accumulation (D_1-4=0.146 5-0.268 8, D_5-8=0.2223-0.345 7). This study explored the regularity and difference of fluorine accumulation in tea cell walls from the perspectives of cultivar and leaf maturity, and provided insights for molecular breeding of low-fluorine tea cultivars.

Key words： tea cultivar; fluorine; leaf cell wall; maturity

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