金萱炒青绿茶中挥发性对映异构体分析方法的优化及应用

张淑怡; 马成英; 陈维; 苗爱清; 乔小燕; 林冬纯; 夏红玲; 许靖逸

doi:10.13305/j.cnki.jts.2024.06.006

茶叶科学 >

2024 , Vol. 44 >Issue 6: 973 - 984

DOI: https://doi.org/10.13305/j.cnki.jts.2024.06.006

研究报告

金萱炒青绿茶中挥发性对映异构体分析方法的优化及应用

张淑怡 ,
马成英 ,
陈维 ,
苗爱清 ,
乔小燕 ,
林冬纯 ,
夏红玲 ,
许靖逸

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1.四川农业大学园艺学院精制川茶四川省重点实验室,四川成都 611130;
2.广东省农业科学院茶叶研究所广东省茶树资源创新利用重点实验室,广东广州 510640

张淑怡,女,硕士研究生,主要从事茶叶加工理论与技术方面的研究。

收稿日期: 2024-08-17

修回日期: 2024-10-30

网络出版日期: 2025-01-08

基金资助

四川省自然科学基金（2023NSFSC0165）、广州市科技计划项目（2023A04J0815）、广东省重点领域研发计划资助（2023B0202120001）、广东省农业科学院科技人才引进专项资金项目（R2021YJ-YB3025）

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Optimization and Application of Analysis Method for Volatile Enantiomers in ‘Jinxuan’ Roasted Green Tea

ZHANG Shuyi ,
MA Chengying ,
CHEN Wei ,
MIAO Aiqing ,
QIAO Xiaoyan ,
LIN Dongchun ,
XIA Hongling ,
XU Jingyi

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1. College of Horticulture, Sichuan Agricultural University, Tea Refining and Innovation Key Laboratory of Sichuan Province, Chengdu 611130, China;
2. Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou 510640, China

Received date: 2024-08-17

Revised date: 2024-10-30

Online published: 2025-01-08

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摘要

为探究挥发性对映异构体在炒青绿茶干燥过程中的变化规律,以金萱炒青绿茶为研究对象,优化了基于顶空固相微萃取-对映选择性-气相色谱-质谱联用技术（Headspace solid phase microextraction-enantioselective-gas chromatography-mass spectrometry,HS-SPME-Es-GC-MS）分析挥发性对映异构体的方法,对120 ℃不同干燥时间（30、60、90 min和120 min）处理的4个炒青绿茶样品进行了对映异构体定性定量分析和化学计量学分析。结果显示,HS-SPME的最佳条件为添加NaCl（3 mL,3 mol·L^-1,料液体积比为1︰6）、萃取温度60 ℃、萃取时间25 min。Es-GC-MS的最佳升温程序为初始温度35 ℃,保持2 min;以4 ℃·min^-1升温至110 ℃,保持10 min;以4 ℃·min^-1升温至210 ℃。采用优化方法分析4个炒青绿茶样品,共检出8种对映异构体,且种类不随干燥时间的延长而发生改变,其中R-(-)-芳樟醇、S-(+)-芳樟醇含量随干燥进程增加,其余6种对映异构体含量在超过60 min或90 min时开始下降。层次聚类分析和偏最小二乘法判别分析结果显示,不同干燥时间样品被明显分成4组,根据变量投影重要性（Variable importance in projection,VIP）>1和差异显著性分析筛选出4种差异对映异构体,按VIP值从大到小分别为R-(-)-芳樟醇、(2R,5S)-茶螺烷B、(2R,5R)-茶螺烷A、S-(+)-芳樟醇。本研究可为炒青绿茶干燥过程中香气品质的提升及干燥工艺选择提供参考依据。

关键词： 炒青绿茶; 顶空固相微萃取; 对映选择性-气相色谱-质谱联用技术; 挥发性成分; 对映异构体

本文引用格式

张淑怡 , 马成英 , 陈维 , 苗爱清 , 乔小燕 , 林冬纯 , 夏红玲 , 许靖逸 . 金萱炒青绿茶中挥发性对映异构体分析方法的优化及应用[J]. 茶叶科学, 2024 , 44(6) : 973 -984 . DOI: 10.13305/j.cnki.jts.2024.06.006

Abstract

To investigate the changes in volatile enantiomers during the drying of ‘Jinxuan’ roasted green tea, an optimized method using headspace solid-phase microextraction-enantioselective-gas chromatography-mass spectrometry (HS-SPME-Es-GC-MS) was applied for qualitative and quantitative enantiomer analysis. The chemometric evaluation was also performed on four roasted green tea samples dried at 120 ℃ for different durations (30 min, 60 min, 90 min and 120 min). The optimal HS-SPME conditions were found to be: addition of NaCl (3 mL, 3 mol·L^-1, solid-to-liquid ratio of 1∶6, extraction temperature of 60 ℃, and extraction time of 25 min. The optimal temperature program for Es-GC-MS was as follows: initial temperature at 35 ℃ held for 2 min, then ramped up to 110 ℃ at 4 ℃ min^-1 and held for 10 min, followed by a further increase to 210 ℃ at 4 ℃ min^-1. Using an optimized method to analyze four ‘Jinxuan’ roasted green tea samples, a total of 8 enantiomers were detected, with no change in the types as drying time increased. Among them, the contents of R-(-)-linalool and S-(+)-linalool increased with the drying process, while the contents of the other 6 enantiomers began to decline after 60 min or 90 min. Hierarchical cluster analysis and partial least squares discrimination analysis show that the samples with different drying times were obviously classified into four groups. Based on variable importance in projection (VIP) >1 and significance analysis, four different enantiomers were screened, and their VIP values were ranked in descending order as R-(-)-linalool, (2R,5S)-theaspirane B, (2R,5R)-theaspirane A, and S-(+)-linalool. This study provided a reference for improving aroma quality and selecting drying processes during the roasting of green tea.

Key words： roasted green tea; HS-SPME; Es-GC-MS; volatile components; enantiomers

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