Based on the previous transcriptome data of the research group, three genes, CsDREB11, CsDREB15, and CsDREB25, were screened. Verified by RT-qPCR, their expressions were induced by drought. The phylogenetic tree, physical and chemical properties, hydrophilicity/hydrophobicity, secondary and tertiary structures, and subcellular localization were predicted and analyzed. The results show that CsDREB11, CsDREB15, and CsDREB25 are most closely related to their homolog genes in Camellia lanceoleosa. They are all unstable hydrophilic proteins. Random coils and α-helices dominate the secondary/tertiary structures. Their subcellular localizations are located in the nucleus. The preliminary study of the subject found that both CsDREB25 and CsPOD3 were highly expressed and significantly correlated under drought conditions, suggesting that there was an interaction between them. Through Y1H, DLA, and LCI experiments, it was verified that CsDREB25 can positively regulate the expression of CsPOD3, increase the activity of POD, remove excessive reactive oxygen species, and improve the drought tolerance of tea plants.
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