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茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应

  • 李悦欣 ,
  • 鄢东海 ,
  • 张金峰 ,
  • 蒲运丹 ,
  • 李帅 ,
  • 孟泽洪
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  • 1.贵州省茶叶研究所,贵州 贵阳 550006;
    2.贵州省茶叶产业技术体系绿色防控与质量安全功能实验室,贵州 贵阳 550006
李悦欣,女,助理研究员,主要从事植物基因工程研究,18786065261@163.com。

收稿日期: 2024-11-15

  修回日期: 2024-12-17

  网络出版日期: 2025-04-30

基金资助

贵州省科技计划项目(黔科合基础-ZK[2024]一般546)、贵州省山地农业关键核心技术攻关项目(GZNYGJHX-2023009)、黔农科博士基金[2025]06号

Identification of the L-type Lectin Receptor Kinase Gene Family in Camellia sinensis and Its Response to Tea Brown Blight and Tea Anthracnose

  • LI Yuexin ,
  • YAN Donghai ,
  • ZHANG Jinfeng ,
  • PU Yundan ,
  • LI Shuai ,
  • MENG Zehong
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  • 1. Tea Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
    2. Green Control and Quality Safety Functional Laboratory of Guizhou Modern Agricultural Industry Technology System of Tea, Guiyang 550006, China

Received date: 2024-11-15

  Revised date: 2024-12-17

  Online published: 2025-04-30

摘要

植物L型凝集素受体激酶(LecRKs)是植物受体激酶家族的重要亚家族,在植物发育和免疫反应中发挥关键作用。本研究系统鉴定并分析了茶树L型凝集素受体激酶(CsLecRKs)基因家族,探讨其在茶树生长发育及抗病防御中的潜在功能。通过HMMsearch分析,共鉴定出59个含有PF00069结构域和PF00139结构域的L型CsLecRKs。基于系统发育分析将其分为5个亚组,不同亚组在外显子-内含子结构上存在显著差异。启动子区域富含与激素响应和逆境胁迫相关的顺式作用元件。转录组数据分析表明,L型CsLecRKs在茶树不同器官中呈现表达差异。qRT-PCR结果显示,CsLecRK IV.1.2CsLecRK S.5在茶炭疽病侵染早期以及茶轮斑病侵染中后期均显著上调表达,且在抗茶炭疽病茶树品种‘黔湄419’中的表达水平显著高于易感品种‘黔湄818’,表明其在茶树对病原菌侵染的免疫反应中发挥关键作用。本研究揭示了L型CsLecRKs在茶树对茶轮斑病和茶炭疽病防御中的关键机制,明确了CsLecRK IV.1.2CsLecRK S.5在病害侵染过程中的表达特征,证实其具有通过激活茶树免疫系统增强植株抗病能力的潜力。研究结果为茶树抗病分子育种和绿色病害防控策略提供了重要的基因资源和理论依据。

本文引用格式

李悦欣 , 鄢东海 , 张金峰 , 蒲运丹 , 李帅 , 孟泽洪 . 茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应[J]. 茶叶科学, 2025 , 45(2) : 253 -265 . DOI: 10.13305/j.cnki.jts.2025.02.001

Abstract

L-type lectin receptor-like kinases (LecRKs) constitute a significant subfamily of plant receptor-like kinases, playing pivotal roles in plant development and immune responses. In this study, the CsLecRK gene family was systematically identified and analyzed in Camellia sinensis to explore their functions in growth, development and disease resistance. A total of 59 L-type CsLecRKs containing PF00069 and PF00139 domains were identified through HMMsearch analysis. Phylogenetic analysis shows these genes could be classified into 5 subgroups, revealing notable differences in exon-intron structures among the subgroups. Promoter region analysis shows enrichment of cis-acting elements associated with hormone responses and stress signaling pathways. Transcriptome data reveals that L-type CsLecRKs exhibited differential expressions in different tea plant tissues. qRT-PCR validation demonstrates that CsLecRK IV.1.2 and CsLecRK S.5 were significantly upregulated during the early stage of Colletotrichum camelliae infection and the later stages of Pestalotiopsis spp. infection. Notably, their expression levels were substantially higher in the resistant cultivar ‘Qianmei 419’ compared to the susceptible cultivar ‘Qianmei 818’, indicating their critical involvement in the immune response to pathogen infection. This study highlighted the critical mechanisms of L-type CsLecRKs in tea plant defenses against brown blight and anthracnose, elucidated the expression profiles of CsLecRK IV.1.2 and CsLecRK S.5 during pathogen infection, and demonstrated their potential to enhance disease resistance by activating the tea plant immune system. These findings provided valuable genetic resources and theoretical support for molecular breeding as well as green disease control strategies in tea plants.

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