茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应

李悦欣; 鄢东海; 张金峰; 蒲运丹; 李帅; 孟泽洪

doi:10.13305/j.cnki.jts.2025.02.001

茶叶科学 >

2025 , Vol. 45 >Issue 2: 253 - 265

DOI: https://doi.org/10.13305/j.cnki.jts.2025.02.001

研究报告

茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应

李悦欣 ,
鄢东海 ,
张金峰 ,
蒲运丹 ,
李帅 ,
孟泽洪

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1.贵州省茶叶研究所,贵州贵阳 550006;
2.贵州省茶叶产业技术体系绿色防控与质量安全功能实验室,贵州贵阳 550006

李悦欣,女,助理研究员,主要从事植物基因工程研究,18786065261@163.com。

收稿日期: 2024-11-15

修回日期: 2024-12-17

网络出版日期: 2025-04-30

基金资助

贵州省科技计划项目（黔科合基础-ZK[2024]一般546）、贵州省山地农业关键核心技术攻关项目（GZNYGJHX-2023009）、黔农科博士基金[2025]06号

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Identification of the L-type Lectin Receptor Kinase Gene Family in Camellia sinensis and Its Response to Tea Brown Blight and Tea Anthracnose

LI Yuexin ,
YAN Donghai ,
ZHANG Jinfeng ,
PU Yundan ,
LI Shuai ,
MENG Zehong

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1. Tea Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
2. Green Control and Quality Safety Functional Laboratory of Guizhou Modern Agricultural Industry Technology System of Tea, Guiyang 550006, China

Received date: 2024-11-15

Revised date: 2024-12-17

Online published: 2025-04-30

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摘要

植物L型凝集素受体激酶（LecRKs）是植物受体激酶家族的重要亚家族,在植物发育和免疫反应中发挥关键作用。本研究系统鉴定并分析了茶树L型凝集素受体激酶（CsLecRKs）基因家族,探讨其在茶树生长发育及抗病防御中的潜在功能。通过HMMsearch分析,共鉴定出59个含有PF00069结构域和PF00139结构域的L型CsLecRKs。基于系统发育分析将其分为5个亚组,不同亚组在外显子-内含子结构上存在显著差异。启动子区域富含与激素响应和逆境胁迫相关的顺式作用元件。转录组数据分析表明,L型CsLecRKs在茶树不同器官中呈现表达差异。qRT-PCR结果显示,CsLecRK IV.1.2和CsLecRK S.5在茶炭疽病侵染早期以及茶轮斑病侵染中后期均显著上调表达,且在抗茶炭疽病茶树品种‘黔湄419’中的表达水平显著高于易感品种‘黔湄818’,表明其在茶树对病原菌侵染的免疫反应中发挥关键作用。本研究揭示了L型CsLecRKs在茶树对茶轮斑病和茶炭疽病防御中的关键机制,明确了CsLecRK IV.1.2和CsLecRK S.5在病害侵染过程中的表达特征,证实其具有通过激活茶树免疫系统增强植株抗病能力的潜力。研究结果为茶树抗病分子育种和绿色病害防控策略提供了重要的基因资源和理论依据。

关键词： 茶树; L型凝集素受体激酶; 茶炭疽病; 茶轮斑病; 免疫反应

本文引用格式

李悦欣 , 鄢东海 , 张金峰 , 蒲运丹 , 李帅 , 孟泽洪 . 茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应[J]. 茶叶科学, 2025 , 45(2) : 253 -265 . DOI: 10.13305/j.cnki.jts.2025.02.001

Abstract

L-type lectin receptor-like kinases (LecRKs) constitute a significant subfamily of plant receptor-like kinases, playing pivotal roles in plant development and immune responses. In this study, the CsLecRK gene family was systematically identified and analyzed in Camellia sinensis to explore their functions in growth, development and disease resistance. A total of 59 L-type CsLecRKs containing PF00069 and PF00139 domains were identified through HMMsearch analysis. Phylogenetic analysis shows these genes could be classified into 5 subgroups, revealing notable differences in exon-intron structures among the subgroups. Promoter region analysis shows enrichment of cis-acting elements associated with hormone responses and stress signaling pathways. Transcriptome data reveals that L-type CsLecRKs exhibited differential expressions in different tea plant tissues. qRT-PCR validation demonstrates that CsLecRK IV.1.2 and CsLecRK S.5 were significantly upregulated during the early stage of Colletotrichum camelliae infection and the later stages of Pestalotiopsis spp. infection. Notably, their expression levels were substantially higher in the resistant cultivar ‘Qianmei 419’ compared to the susceptible cultivar ‘Qianmei 818’, indicating their critical involvement in the immune response to pathogen infection. This study highlighted the critical mechanisms of L-type CsLecRKs in tea plant defenses against brown blight and anthracnose, elucidated the expression profiles of CsLecRK IV.1.2 and CsLecRK S.5 during pathogen infection, and demonstrated their potential to enhance disease resistance by activating the tea plant immune system. These findings provided valuable genetic resources and theoretical support for molecular breeding as well as green disease control strategies in tea plants.

Key words： tea plant; L-type lectin receptor kinases; tea anthracnose; tea brown blight; immune response

参考文献

[1] 蔡晓明, 边磊, 罗宗秀, 等. 2023年茶树病虫害防控研究进展[J]. 中国茶叶, 2024, 46(10): 1-7.
Cai X M, Bian L, Luo Z X, et al.Research progress of tea pest control in 2023[J]. China Tea, 2024, 46(10): 1-7.
[2] 程开鑫, 杨凯欣, 邓雅元, 等. 山茶炭疽菌对茶树的致病性及其对杀菌剂的敏感性研究[J]. 茶叶科学, 2023, 43(1): 55-66.
Cheng K X, Yang K X, Deng Y Y, et al.Pathogenicity and fungicide sensitivity of Colletotrichum camelliae from tea plant (Camellia sinensis)[J]. Journal of Tea Science, 2023, 43(1): 55-66.
[3] 赵永田, 马悦, 胡德禹, 等. 我国茶树叶部主要真菌病害绿色防控现状与展望[J]. 植物保护, 2023, 49(5): 133-144.
Zhao Y T, Ma Y, Hu D Y, et al.Current status and prospects of green control for major fungal diseases of tea leaves in China[J]. Plant Protection, 2023, 49(5): 133-144.
[4] 陈荣冰, 罗宗秀, 王丽丽, 等. 茶树主要病虫害绿色防控技术[J]. 茶叶科学, 2021, 41(3): 265-274.
Chen R B, Luo Z X, Wang L L, et al.Green control techniques for major tea plant diseases and pests[J]. Journal of Tea Science, 2021, 41(3): 265-274.
[5] 郑世仲, 周子维, 陈晓慧, 等. 拮抗炭疽病的茶树内生菌筛选、鉴定及培养条件优化[J]. 茶叶科学, 2023, 43(2): 205-215.
Zheng S Z, Zhou Z W, Chen X H, et al.Screening, identification and culture condition optimization of antagonistic endophytic bacteria against Gloeosporium theae-sinensis Miyake[J]. Journal of Tea Science, 2023, 43(2): 205-215.
[6] 刘艳潇, 祝一鸣, 周而勋. 植物免疫诱抗剂的作用机理和应用研究进展[J]. 分子植物育种, 2020, 18(43): 1020-1026.
Liu Y X, Zhu Y M, Zhou E X.Research progress on the action mechanism and application of plant immune inducers[J]. Molecular Plant Breeding, 2020, 18(43): 1020-1026.
[7] Sun Y L, Qiao Z Z, Muchero W, et al.Lectin receptor-like kinases: the sensor and mediator at the plant cell surface[J]. Frontiers in Plant Science, 2020, 11: 596301. doi: 10.3389/fpls.2020.596301.
[8] Wang Y, Bouwmeester K.L-type lectin receptor kinases: new forces in plant immunity[J]. PLoS Pathog, 2017, 13(8): e1006433. doi: 10.1371/journal.ppat.1006433.
[9] Hervé C, Dabos P, Galaud J P, et al.Characterization of an Arabidopsisth aliana gene that defines a new class of putative plant receptor kinase with an extracellular lectin-like domain[J]. Journal of Molecular Biology, 1996, 258(5): 778-788.
[10] Barre A, Hervé C, Lescure B, et al.Lectin receptor kinases in plants[J]. Critical Reviews in Plant Sciences, 2002, 21(6): 379-399.
[11] Wang Y, Nsibo D L, Juhar H M, et al.Ectopic expression of Arabidopsis L-type lectin receptor kinase genes LecRK-I.9 and LecRK-IX.1 in Nicotiana benthamiana confers phytophthora resistance[J]. Plant Cell Reports, 2016, 35(4): 845-855.
[12] Woo J Y, Kim Y J, Paek K H.CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response[J]. Biochemical and Biophysical Research Communications, 2020, 524(4): 951-956.
[13] Sun E, Yu H Q, Chen Z J, et al.Enhanced Valsa canker resistance conferred by expression of MdLecRK-S.4.3 in Pyrus betulifolia is largely suppressed by PbePUB36[J]. Journal of Experimental Botany, 2023, 165(1): 145-155.
[14] Cheng X Y, Wu Y, Guo J P, et al.A rice lectin receptor-like kinase that is involved in innate immune responses also contributes to seed germination[J]. The Plant Journal, 2013, 76(4): 687-698.
[15] Xia E H, Li F D, Tong W, et al.Tea plant information archive: a comprehensive genomics and bioinformatics platform for tea plant[J]. Plant Biotechnology Journal, 2019, 17(10): 1938-1953.
[16] Finn R D, Clements J, Eddy S R.HMMER web server: interactive sequence similarity searching[J]. Nucleic Acids Research, 2011, 39(s2): W29-W37.
[17] Finn R D, Coggill P, Eberhardt R Y, et al.The Pfam protein families database: towards a more sustainable future[J]. Nucleic Acids Research, 2016, 44(D1): D279-D285.
[18] Ivica L, Peer B.20 years of the SMART protein domain annotation resource[J]. Nucleic Acids Research, 2018, 46(D1): D493-D496.
[19] 谢道龙, 邹肖肖, 李美玲, 等. LecRKⅢ.2基因调控拟南芥对非生物胁迫和外源激素的响应[J]. 广西师范大学学报(自然科学版), 2023, 41(4): 189-199.
Xie D L, Zou X X, Li M L, et al.LecRKIII.2 gene regulates response of Arabidopsis thaliana to abiotic stress and exogenous hormones[J]. Journal of Guangxi Normal University (Natural Science Edition), 2023, 41(4): 189-199.
[20] Wang Y, Cordewener J H, America A H, et al.Arabidopsis lectin receptor kinases LecRK-IX.1 and LecRK-IX.2 are functional analogs in regulating Phytophthora resistance and plant cell death[J]. Molecular Plant-Microbe Interactions, 2015, 28(9): 1032-1048.
[21] Yekondi S, Liang F C, Okuma E, et al.Nonredundant functions of Arabidopsis LecRK-V.2 and LecRK-VII.1 in controlling stomatal immunity and jasmonate-mediated stomatal closure[J]. New Phytologist, 2018, 218(1): 253-268.
[22] Wang Y, Bouwmeester K, Beseh P, et al.Phenotypic analyses of Arabidopsis T-DNA insertion lines and expression profiling reveal that multiple L-type lectin receptor kinases are involved in plant immunity[J]. Molecular Plant-Microbe Interactions, 2014, 27(12): 1390-1402.
[23] 彭小群, 邹雅琦, 骆素微, 等. 植物凝集素类受体激酶参与抗病的研究进展[J]. 植物科学学报, 2022, 40(1): 105-114.
Peng X Q, Zou Y Q, Luo S W, et al.Research progress on lectin receptor-like kinases and their roles in mediation of plant disease resistance[J]. Plant Science Journal, 2022, 40(1): 105-114.
[24] Bellande K, Bono J J, Savelli B, et al.Plant lectins and lectin receptor-like kinases: how do they sense the outside?[J]. International Journal of Molecular Science, 2017, 18(6): 1164. doi: 10.3390/ijms18061164.
[25] Guo L, Qi Y T, Mu Y, et al. Potato StLecRK-IV.1 negatively regulates late blight resistance by affecting the stability of a positive regulator StTET8[J]. Horticulture Research, 2022, 9: uhac010. doi: 10.1093/hr/uhac010.
[26] Wu T Q, Wang R, Xu X M, et al.Cucumis sativus L-type lectin receptor kinase (CsLecRK) gene family response to Phytophthora melonis, Phytophthora capsici and water immersion in disease resistant and susceptible cucumber cultivars[J]. Gene, 2014, 549(2): 214-222.
[27] Pham A Q, Cho S H, Nguyen C T, et al.Arabidopsis lectin receptor kinase P2K2 is a second plant receptor for extracellular ATP and contributes to innate immunity[J]. Plant Physiology, 2020, 183(3): 1364-1375.
[28] Wang Y, Weide R, Govers F, et al.L-type lectin receptor kinases in Nicotiana benthamiana and tomato and their role in Phytophthora resistance[J]. Journalof Experimental Botany, 2015, 66(21): 6731-6743.
[29] Wang C G, Huang X E, Li Q, et al.Extracellular pyridine nucleotides trigger plant systemic immunity through a lectin receptor kinase/BAK1 complex[J]. Nature Communications, 2019, 10: 4810. doi: 10.1038/s41467-019-12781-7.

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