茶树叶斑病病原菌的分离与鉴定

杨芳; 江冰冰; 雷金梅; 郭存武; 李丽梅; 徐嘉忆; 王兴华; 袁文侠; 王白娟

doi:10.13305/j.cnki.jts.2025.02.006

茶叶科学 >

2025 , Vol. 45 >Issue 2: 266 - 272

DOI: https://doi.org/10.13305/j.cnki.jts.2025.02.006

研究报告

茶树叶斑病病原菌的分离与鉴定

杨芳 ,
江冰冰 ,
雷金梅 ,
郭存武 ,
李丽梅 ,
徐嘉忆 ,
王兴华 ,
袁文侠 ,
王白娟

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1.云南农业大学茶学院云南省高校智能有机茶园建设重点实验室,云南昆明 650201;
2.云南农业大学云南有机茶产业智能工程研究中心,云南昆明 650201;
3.云南农业大学植物保护学院农业生物多样性应用技术国家工程研究中心,云南昆明 650201

杨芳,硕士研究生,主要从事茶树病害生物防治方面的研究。

收稿日期: 2024-12-16

修回日期: 2025-02-19

网络出版日期: 2025-04-30

基金资助

云南省乡村振兴科技专项（202304BI090013）、云南省重大科技专项计划（202302AE09002001）、云南省教育厅科学研究基金项目青年人才基础研究（2024J0406）、云南省建设面向南亚东南亚科技创新中心专项（202403AP140022）

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Isolation and Identification of the Pathogen Causing Leaf Spot in Tea Plants

YANG Fang ,
JIANG Bingbing ,
LEI Jinmei ,
GUO Cunwu ,
LI Limei ,
XU Jiayi ,
WANG Xinghua ,
YUAN Wenxia ,
WANG Baijuan

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1. College of Tea Science, Yunnan Agricultural University Key Laboratory of Intelligent Organic Tea Garden Construction in Universities of Yunnan Province, Kunming 650201, China;
2. Yunnan Organic Tea Industry Intelligent Engineering Research Center, Yunnan Agricultural University, Kunming 650201, China;
3. National Engineering Research Center for Applied Technology of Agricultural Biodiversity, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China

Received date: 2024-12-16

Revised date: 2025-02-19

Online published: 2025-04-30

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摘要

茶树[Camellia sinensis (L.) O. Kuntze]是我国重要的经济作物。叶斑病是茶树重要的叶部病害之一,严重影响茶叶的产量和品质。2023年,在云南省西双版纳傣族自治州勐海县茶园进行病害调查时发现茶园茶树30%的叶片出现类似茶树叶斑病的症状,为明确该病原,采用组织分离法和有伤接种法对病原菌进行分离和致病性测定,分别观察形态学特征并结合分子生物学技术确定病原菌的种类。结果表明,分离的3个菌株均能够侵染茶树叶片,是茶树叶斑病的致病菌;根据其形态学特征并结合多基因（ITS、TUB2和TEF-1α）系统发育分析结果确定引起茶树叶斑病的病原菌为Nigrospora musae。研究结果可为该病菌引起的茶树叶斑病的防控提供理论基础。

关键词： 茶树; 叶斑病; 黑孢菌; 病原鉴定

本文引用格式

杨芳 , 江冰冰 , 雷金梅 , 郭存武 , 李丽梅 , 徐嘉忆 , 王兴华 , 袁文侠 , 王白娟 . 茶树叶斑病病原菌的分离与鉴定[J]. 茶叶科学, 2025 , 45(2) : 266 -272 . DOI: 10.13305/j.cnki.jts.2025.02.006

Abstract

Tea plant [Camellia sinensis (L.) O. Kuntze] is an important agricultural economic crop in China. Tea leaf spot disease is one of the most important leaf diseases of tea plants. It seriously affects the yield and quality of tea. In 2023, a tea leaf spot disease was found in a tea garden in Menghai County, Xishuangbanna Dai Autonomous Prefecture Yunnan Province. In order to clarify the pathogen, the tissue isolation method and wound inoculation method were used to isolate and determine the pathogenicity of the pathogen. The morphological characteristics were observed and combined with molecular biology techniques to determine the type of pathogen. The results show that the three strains could infect tea leaves and were the causal agents of tea leaf spot. The pathogen causing tea leaf spot was identified as Nigrospora musae by observing its morphological characteristics and phylogenetic analysis of multiple genes (ITS, TUB2 and TEF-1α). The results of this study provided a theoretical basis for the prevention and control of tea leaf spot caused by the pathogen.

Key words： Camellia sinensis; leaf spot disease; Nigrospora musae; pathogen identification

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