茶树SRO基因家族的鉴定及表达分析

郭永春; 王鹏杰; 陈笛; 郑玉成; 陈雪津; 叶乃兴

doi:10.13305/j.cnki.jts.2019.04.004

茶叶科学 >

2019 , Vol. 39 >Issue 4: 392 - 402

DOI: https://doi.org/10.13305/j.cnki.jts.2019.04.004

茶树SRO基因家族的鉴定及表达分析

郭永春 ,
王鹏杰 ,
陈笛 ,
郑玉成 ,
陈雪津 ,
叶乃兴

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福建农林大学园艺学院/茶学福建省高校重点实验室,福建福州 350002

郭永春,女,硕士研究生,主要从事茶树栽培育种与生物技术研究方面的研究。

收稿日期: 2019-02-11

网络出版日期: 2019-08-19

基金资助

福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项（闽教科〔2015〕75号）、国家现代农业（茶叶）产业技术体系建设专项资金项目（CARS-19）、福建农林大学科技创新专项基金项目（CXZX2016117、CXZX2017181）

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Genome-wide Identification and Expression Analysis of SRO Gene Family in Camellia sinensis

GUO Yongchun ,
WANG Pengjie ,
CHEN Di ,
ZHENG Yucheng ,
CHEN Xuejin ,
YE Naixing

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College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science at Universities in Fujian, Fuzhou 350002, China

Received date: 2019-02-11

Online published: 2019-08-19

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摘要

SROs（Similar to rcd one）是植物特有的基因家族。本研究利用生物信息学方法从茶树基因组中鉴定获得9个茶树SRO基因家族成员,分别命名为CsRCD1—4和CsSRO1—5。9个茶树SRO基因的编码蛋白均具有特征结构域PARP和RST,具有相似的保守基序。系统进化树分析聚分为3组,Ι组包含CsRCD1—4,Ⅱ组包含CsSRO1和CsSRO2,Ⅲ组包含CsSRO3—5。基因结构分析表明每个CsSRO基因含有4至9个外显子。8个茶树组织转录组数据分析表明,CsRCD1、CsRCD3和CsRCD4可能在茶树不同发育阶段具有重要作用;大多数CsSRO基因在根和成熟叶中较高表达。上游启动子区域分析发现大量与植物发育、激素及胁迫响应密切相关的顺式作用元件,进一步对CsSRO基因在干旱和脱落酸处理下的表达模式进行分析发现,9个CsSRO基因均被诱导表达,CsSRO基因可能与茶树抗旱密切相关。

关键词： 茶树; SRO; 系统进化; 干旱胁迫

本文引用格式

郭永春 , 王鹏杰 , 陈笛 , 郑玉成 , 陈雪津 , 叶乃兴 . 茶树SRO基因家族的鉴定及表达分析[J]. 茶叶科学, 2019 , 39(4) : 392 -402 . DOI: 10.13305/j.cnki.jts.2019.04.004

Abstract

SROs (Similar to rcd one) are plant-specific gene families. In this study, 9 CsSRO gene family members were identified from tea tree genome by bioinformatics method and named as CsRCD1—4 and CsSRO1—5 respectively. All coding proteins of the 9 CsSRO genes have characteristic structural domains PARP and RST, and have similar conserved motifs. The CsSRO genes were divided into 3 groups based on phylogenetic tree analysis, with the group Ι containing CsRCD1—4, the group Ⅱ containing CsSRO1, CsSRO2 and the group Ⅲ containing CsSRO3—5. Gene structure analysis shows that this gene family contained 4 to 9 exons. Analysis of transcriptome data from 8 tea tree tissues shows that CsRCD1/CsRCD3/CsRCD4 might play an important role in different developmental stages of tea plants. Most CsSRO genes were highly expressed in roots and mature leaves. Upstream promoter region analysis found a large number of cis-acting elements closely related to plant development, hormones and stress response. Further expression analysis shows that 9 CsSRO genes were induced by drought and abscission acid treatments, suggesting CsSRO genes may be closely related to drought resistance.

Key words： Camellia sinensis; SRO; phylogeny analysis; stress

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