采用菌丝生长速率法、Horsfall方法和共毒系数法测定了香茅醇不同构型对茶炭疽病病菌(Colletotrichum gloeosporioides)的毒力以及两构型不同比例混配的协同效应。结果表明,右旋香茅醇和左旋香茅醇对茶树炭疽病菌的EC50分别为(113.27±0.95) mg·L-1和(119.87±0.20) mg·L-1。按照质量比1.6︰1将右旋香茅醇和左旋香茅醇混配,协同增效作用最高,共毒系数为130.19;质量比为1︰1.4和1︰3.8时,共毒系数分别为120.57和121.42,也具有协同增效作用。结果表明,香茅醇及两旋光异构体对抑制茶炭疽病菌具有良好的活性,将两者按一定比例混用后具有增效作用。
In this study, the activities of dextral citronellol, rhodinol and their synergistic effect to the C. gloeosporioides was determined by method of mycelial growth rate, Horsfall and co-toxicity coefficient. The EC50 of dextral citronellol and rhodinol were (113.27±0.95) mg·L-1 and (119.87±0.20) mg·L-1 respectively. The dextral citronellol and rhodinol were mixed according to the mass ratio. The result shows that the synergistic effect was the highest with the co-toxicity coefficient of 130.19 under the mass ratio of 1.6︰1 (dextral citronellol︰rhodinol). The co-toxicity coefficients were 120.57 and 121.42 when the mass ratio were 1︰1.4 and 1︰3.8, respectively. The results indicate that citronellol and its two optical isomers had high inhibiting activities against C. gloeosporioides, and the synergistic effect could be performed by mixing them within certain proportion.
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