一株拮抗茶炭疽病菌的木霉菌的分离、筛选及鉴定

赵兴丽; 张金峰; 周玉锋; 赵玳琳; 张莉; 周罗娜; 陶刚

doi:10.13305/j.cnki.jts.2019.04.008

茶叶科学 >

2019 , Vol. 39 >Issue 4: 431 - 439

DOI: https://doi.org/10.13305/j.cnki.jts.2019.04.008

一株拮抗茶炭疽病菌的木霉菌的分离、筛选及鉴定

赵兴丽 ,
张金峰 ,
周玉锋 ,
赵玳琳 ,
张莉 ,
周罗娜 ,
陶刚

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1. 贵州省农业科学院农业生物技术重点实验室,贵州贵阳 550006;
2. 贵州省农业科学院茶叶研究所,贵州贵阳 550006;
3. 贵州省农业科学院植物保护研究所,贵州贵阳 550006;
4. 贵州省农业科学院草业研究所,贵州贵阳 550006

赵兴丽,女,硕士,主要从事植物真菌病害的防治方面的研究。

收稿日期: 2018-12-06

网络出版日期: 2019-08-19

基金资助

国家现代农业（茶叶）产业技术体系建设专项资金项目（CARS-19）、国家重点研发计划项目子课题（2016YFD0200906、2017YFD0201102）、黔科合平台人才[2017]5717号、黔农科院自主创新科研专项字[2014]009号

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Isolation, Screening and Identification of A Strain of Trichoderma Antagonizing Tea Anthracnose

ZHAO Xingli ,
ZHANG Jinfeng ,
ZHOU Yufeng ,
ZHAO Dailin ,
ZHANG Li ,
ZHOU Luona ,
TAO Gang

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1. Guizhou Academy of Agricultural Sciences key laboatory of agricultural biotechnology, Guiyang 550006, China;
2. Guizhou Academy of Agricultural Sciences tea research institute, Guiyang 550006, China;
3. Guizhou Academy of Agricultural Sciences institute of Plant Protection, Guiyang 550006, China;
4. Guizhou Academy of Agricultural Sciences grass industry research institute, Guiyang 550006, China

Received date: 2018-12-06

Online published: 2019-08-19

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摘要

为获得对茶炭疽病有生物防治效果的拮抗木霉菌,本文采用梯度稀释法从茶树根际土壤中分离获得23株木霉菌,并通过平板对峙法和抑菌圈法从中筛选出1株强拮抗茶炭疽病菌的木霉菌株LS17110205。该菌株对茶炭疽病菌抑菌率达76.96%,并能在茶炭疽病菌菌落上产生大量白色菌丝及绿色分生孢子,使茶炭疽病菌菌落萎缩,颜色变暗;其发酵液也能有效抑制茶炭疽病菌菌丝生长,抑制率达70.08%。扫描电镜观察发现,菌株LS17110205发酵液能使茶炭疽菌菌丝表面皱缩。基于形态学特征结合分子系统发育树分析,将菌株LS17110205鉴定为Trichoderma asperelloides。该研究结果为茶炭疽病的生物防治提供一定的理论基础。

关键词： 根际土壤; 抑菌率; 茶炭疽病; 鉴定; 木霉菌

本文引用格式

赵兴丽 , 张金峰 , 周玉锋 , 赵玳琳 , 张莉 , 周罗娜 , 陶刚 . 一株拮抗茶炭疽病菌的木霉菌的分离、筛选及鉴定[J]. 茶叶科学, 2019 , 39(4) : 431 -439 . DOI: 10.13305/j.cnki.jts.2019.04.008

Abstract

To obtain antagonistic Trichoderma for biocontrol of tea anthracnose, the twenty-three Trichoderma strains were isolated from Camellia sinensis rhizosphere-soil by taking gradient dilution. Among them, the strain LS17110205 against tea anthracnose was screened by using dual-culture and inhibition zone assay. The results show that the inhibitory rate of LS17110205 against tea anthracnose was up to 76.96% and a large number of white hyphae and green spores were produced on the colony of tea anthracnose, which caused the tea anthracnose colony to shrink and became dark. The fermentation liquid of LS17110205 was effective against pathogen hyphae, and the inhibitory rate was 70.08%. The results show that the fermentation of the strain LS17110205 caused the tea anthracnose mycelia to shrink on the surface by scanning electron microscope analysis. LS17110205 was identified as Trichoderma asperelloides on the basis of the morphological characteristics and phylogenetic tree. The results provided theoretical basis in biocontrol of tea anthracnose.

Key words： rhizosphere-soil; inhibitory rate; tea anthracnose; identification; Trichoderma spp

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