以南京中山陵茶园中十年生龙井长叶茶树为试验材料,用酶联免疫法（ELISA）测定自然低温下茶树叶片内源激素含量的变化,以探讨自然低温下茶树内源激素变化规律。结果显示,自然越冬期间,茶树叶片中吲哚乙酸（IAA）含量的波动幅度最大,脱落酸（ABA）次之,而赤霉素（GA3）与玉米素核苷（ZR）波动幅度较小;同时,除了ABA外,茶树其他内源激素IAA、ZR和GA3含量变化趋势一致;激素间比值变化和相关性分析显示ZR与GA3、ZR与IAA、IAA与GA3含量变化极显著相关,ABA与IAA、ABA与ZR呈显著正相关。试验结果表明,茶树内源激素之间关系密切,茶树可能通过调控ABA/(GA3+IAA+ZR)的比值来适应自然低温环境。
To understand the changes of endogenous hormones in tea plant [Camellia sinensis(L.) O. Kuntze] under natural low-temperature, ten-year-old tea plants of the cultivar ‘Longjingchangye’ were used as the material and the changes of endogenous hormones content were determined by enzyme-linked immunosorbent assay (ELISA). The results showed that the fluctuation amplitude of indole acetic acid (IAA) content is highest among all detected hormones during overwintering period. The change of abscisic acid (ABA) content was smaller than IAA but higher than that of gibberellins (GA3) and zeatinriboside (ZR). In addition, the changing trends of IAA, ZR and GA3 content is consistent, while the trend of ABA is different from them. Furthermore, the changes between ZR and GA3, ZR and IAA, IAA and GA3 content were significantly related. As well, there are significant positive correlations between ABA and IAA, ZR, respectively. All of the above results suggest that endogenous hormones in tea plant are closely related under natural low-temperature. Tea plant may regulate the ratio of ABA/(GA3 + IAA + ZR) to adapt to low temperature.
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