建立一种茶树花多糖含量的修正系数蒽酮-硫酸检测方法。根据质量守恒定律,在普通蒽酮-硫酸检测多糖方法基础上,通过联用离子色谱检测技术,构建蒽酮-硫酸检测新方法的修正系数（f）,实现茶树花多糖检测技术的快捷、准确和客观。研究表明,以半乳糖为标准对照品时修正系数f半乳糖=2.84,以葡萄糖为标准对照品时修正系数f葡萄糖=4.49。该方法稳定性好（RSD葡萄糖=1.7%,RSD半乳糖=1.0%）、精密度高（RSD葡萄糖=1.4%,RSD半乳糖=2.0%）、重复性强（RSD葡萄糖=3.2%,RSD半乳糖=2.5%）及加标回收率佳（回收率葡萄糖=91.3%~104.1%,回收率半乳糖=95.9%~104.4%）。用新方法测得3产地茶树花多糖含量分别为10.30%、10.07%及9.99%,高于常规蒽酮-硫酸法检测茶树花多糖值。茶树花多糖中单糖组分分析表明,以半乳糖为标准对照品检测茶树花多糖较好。
：A method for correction coefficient of anthrone sulfuric acid to detect the polysaccharides in tea flower was established. According to the law of conservation of mass and normal anthrone sulfuric acid method determination of polysaccharide, correction coefficient for a new detection method of anthrone sulfuric acid (f) was constructed through the combined use of ion chromatography technology, which laid the foundation for the tea flower polysaccharide detection technology with fast, accurate and objective charcteristics. The results showed that the correction coefficient were 2.84 and 4.49 when galactose and glucose were used as standard reference substance respectively. The method had good stability (RSDglucose=1.7%, RSDgalactose=1.0%), high precision (RSDglucose=1.4%, RSDgalactose=2.0%), strong repeatability (RSDglucose=3.2%, RSDgalactose=2.5%) and well recovery (recoveryglucose=91.3%-104.1%, recoverygalactose=95.9%-104.4%). Tea flower polysaccharide contents determined by the new method were 10.30%, 10.07% and 9.99% respectively in samples from Anhui, Sichuan and Zhejiang provinces, which were higher than the value determined by conventional anthrone sulfuric acid method. Monosaccharide composition analysis of polysaccharides in tea flower showed that galactose was better to be used as standard reference.
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