茶树修剪物直接还田是茶园管理中常见的栽培措施。本文通过提取不同处理的土壤样品总DNA,以未处理土壤样品为对照,利用变性梯度凝胶电泳（PCR-DGGE）,研究添加茶树修剪物对茶园土壤真菌群落结构多样性的影响。结果显示,各处理土壤样品的DGGE图谱中发现许多公共条带,这些共有条带主要属于Pezizomycotina门、Basidiomycota门、Mortierellomycotina门和Mucoromycotina门;通过DGGE的条带数和亮度进行真菌群落多样性各项指标的比较,显示各处理的丰富度指数和香农-威纳指数均低于对照,表明茶树修剪物的降解产物和茶树本身固有的酚类物质对茶园的真菌群落有一定的抑制作用。
Returning of pruning material to the field is a common agronomic practice performed in tea plantation. By extracting the total DNA of soil in various treatments, the effects of tea pruning material on fungal community structure and diversity in tea garden soils were investigated by using PCR amplification and denaturing gradient gel electrophoresis (DGGE), the original soil without any supplement was set as control. Many common bands were detected from each soil samples by DGGE, and those bands that showed highest sequence homology to Pezizomycotina, Basidiomycota, Mortierellomycotina and Mucoromycotina. The number and brightness of bands from DGGE presented the community diversity of fungi under different treatments. The results showed the richness and Shannon index were lower than the control, maybe the degradation products and phenolic compounds of pruning material have some inhibitory effect on fungi community diversity.
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