通过形态学鉴定和rDNA-ITS序列分析,明确了从广东英德茶园中采集到的一株寄生茶丽纹象甲的白僵菌为球孢白僵菌,并命名为Bb1-1。为明确该菌株的生防潜力,以本实验室保存的2株茶丽纹象甲高毒力球孢白僵菌菌株（XJBb3005和XJBb3008）为参考菌,研究了菌株Bb1-1的生长速率、产孢量、孢子萌发率、耐热力和抗紫外能力等生物学特性,并测定了该菌株在25℃、29℃和32℃下对茶丽纹象甲的毒力。结果表明,3个菌株的生长速率、孢子萌发率和抗紫外能力没有明显的差异;3个菌株产孢量大小顺序为XJBb3005>Bb1-1>XJBb3008;但Bb1-1具有最强的耐热力。在25℃下,3个菌株对茶丽纹象甲成虫的累积校正死亡率之间差异不显著,都超过93.94%;但在高温（29℃和32℃）下,菌株Bb1-1对茶丽纹象甲的杀虫毒力优于两个参考菌株,处理7βd累计校正死亡率分别为90.91%和80%,LT50分别为3.83βd和5.28βd。鉴于菌株Bb1-1耐热力较强,且在高温下对茶丽纹象甲成虫具有较好的杀虫毒力,将在今后该虫的生物防治中发挥重要的作用。
Through morphological characters and rDNA-ITS identification, a Myllocerinus aurolineatus-Beauveria strain that was isolated from tea plantation in Yingde Guangdong Province was identified to be Beauveria bassiana, and named as Bb1-1. In order to confirm the biocontrol potential of Bb1-1, two highly virulent B. bassiana strain towards M. aurolineatus weevil used as the reference, the strain growth rate, sporulation, spore germination rate, thermotolerance and UV resistance of Bb1-1, and the virulence of Bb1-1 to M. aurolineatus weevil under 25℃, 29℃ and 32℃ were tested. The results showed that there were no significant difference on the strain growth rate, spore germination rate, and UV resistance among three strains; the sporulation decreased in this order: XJBb3005> Bb1-1> XJBb3008; however, Bb1-1 showed the best thermotolerance. At 25℃, the cumulative corrective mortality of three strains were all beyond 93.94%, and there were no significance among them. However, the virulence of Bb1-1 to M. aurolineatus weevil was higher than that of the two reference strains under high temperature. At 29℃, the cumulative corrective mortality was 90.91% (7 d), LT50 value was 3.83d. At 32℃, the cumulative corrective mortality was 80% (7 d), LT50 value was 5.28 d. In consideration of Bb1-1’s good thermotolerance and the highly virulent to M. aurolineatus weevil under high temperature, it’ll play a very important role in biocontrol of M. aurolineatus weevil.
[1] 曾明森, 吴光远, 王庆森. 我国茶丽纹象甲的研究进展[J]. 河南科技大学学报: 农学版, 2003, 23(4): 16-20.
[2] 张汉鹄, 谭济才. 中国茶树害虫及其无公害治理[M]. 合肥: 安徽科学技术出版社, 2004: 234-235.
[3] 彭萍, 王晓庆, 李品武. 茶树病虫害测报与防治技术[M]. 北京: 中国农业出版社, 2013: 163-166.
[4] 曾明森, 吴光远. 福建茶树病虫害与天敌图谱[M]. 北京: 中国农业科学技术出版社, 2014: 79-80.
[5] Feng MG, Poprawski TJ, Khachatourians GG.Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status[J]. Biocontrol Science and Technology, 1994, 4: 3-34.
[6] 李增智, 王联德. 昆虫病原真菌的利用[M]//林乃铨. 害虫生物防治. 北京: 科学出版社, 2010: 248-254.
[7] Faria MR de, Wraight SP. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types[J]. Biology Control, 2007, 43: 237-256.
[8] 吴光远, 孙椒德, 林阿祥, 等. 应用白僵菌871菌株防治茶丽纹象甲的研究[J]. 茶叶科学简报, 1991(4): 15-20.
[9] 吴光远, 孙椒德, 曾明森, 等. 白僵菌871菌株防治茶丽纹象甲的效果[J]. 福建农科院学报, 1995, 10(2): 39-43.
[10] 吴光远, 孙椒德, 曾明森, 等. 白僵菌871菌株与菌粉生产工艺研究简报[J]. 茶叶科学简报, 1993(3): 20-22, 15.
[11] 吴光远, 曾明森, 王庆森, 等. 白僵菌871菌株毒理及其防治茶丽纹象甲[J]. 武夷科学, 2002(1): 156-159.
[12] 王定锋, 曾明森, 王庆森, 等. 球孢白僵菌不同分离株生物学特性及对茶丽纹象甲成虫的杀虫活性研究[J]. 福建农业学报, 2012, 27(7): 750-754.
[13] 王定锋, 刘丰静, 李慧玲, 等. 球孢白僵菌XJBb3005对茶丽纹象甲致病力的时间-剂量-死亡率模型分析[J]. 福建农业学报, 2013, 28(8): 807-811.
[14] 唐晓庆, 樊美珍, 李增智. 球孢白僵菌继代培养中菌落局变现象及环境影响因素的研究[J]. 真菌学报, 1996, 15(3): 188-196.
[15] 唐晓庆, 唐燕平, 李增智. 球孢白僵菌菌种退化及其对马尾松毛虫防治的影响[J]. 安徽农业大学学报, 1996, 23(3): 246-253.
[16] 王滨, 樊美珍, 李增智. 球孢白僵菌选择性培养基的筛选[J]. 安徽农业大学学报, 2000, 27(1): 23-28.
[17] 蒲蛰龙, 李增智. 昆虫真菌学[M]. 合肥: 安徽科学技术出版社, 1996: 297-301.
[18] INNIS MA, GELFAND DH, SNINSKY JJ, et al.PCR protocols: a guide to methods and applications [M]. San Diego: Academic Press, 1990: 315-322.
[19] Tamura K, Dudley J, Nei M, et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24: 1596-1599.
[20] 李忠, 金道超, 邹晓, 等. 蝉拟青霉不同来源菌株的生长特性及其对蚜虫的致病性[J]. 植物保护学报, 2007, 34(6): 637-641.
[21] 雷妍圆, 吕利华, 何余容, 等. 球孢白僵菌生物学特性与其对小菜蛾致病力相关性分析[J]. 中国生物防治, 2010, 26(2): 143-148.
[22] 俞佳, 冯明光. 基于分生孢子热胁迫反应的球孢白僵菌耐热菌株筛选[J]. 菌物学报, 2006, 25(2): 278-283.
[23] 彭慧, 郑岳臣. rDNA ITS序列在鉴定尖端赛多孢子菌中的应用[J]. 同济医科大学学报, 2000, 29(1): 26-28.
[24] 何劲, 康冀川, 雷帮星, 等. 四株虫生真菌的鉴定及其对小菜蛾的室内毒力[J]. 植物保护学报, 2010, 37(4): 341-346.
[25] 张建伟, 王中康, 申剑飞, 等. 小菜蛾高致病力绿僵菌的筛选、鉴定及培养特性研究[J]. 中国生物防治学报, 2012, 28(1): 53-61.
[26] 王定锋, 杨广, 王庆森, 等. 两株棒束孢菌的鉴定及其对茶卷叶蛾和茶小卷叶蛾的致病力[J]. 植物保护学报, 2014, 41(5): 531-539.
[27] 李鸿文, 冯明光. 球孢白僵菌不同菌株分生孢子的耐热能力[J]. 浙江大学学报: 农业与生命科学版. 2008, 34(2): 158-162.
[28] Verghese J, Abrams J, Wang Y, et al. Biology of the heat shock response and protein chaperones: budding yeast (Saccharomyces cerevisiae) as a model system[J]. Microbiol Mol Biol Rev, 2012, 76(2): 115-158.
[29] Luz C, Fargues J.Temperature and moisture requirements for conidial germination of an isolate of Beauveria bassiana, Pathogenic to Rhodnius Prolixus[J]. Mycopathologia, 1997, 138: 117-125.
[30] 蒲蛰龙. 昆虫病理学[M]. 广州: 广东科技出版社, 1994: 349.
浙公网安备 33019902000101号 
