外源水杨酸甲酯对高温胁迫下茶树光合作用和抗氧化酶的影响

魏吉鹏; 李鑫; 王朝阳; 李洋; 张兰; 沈晨; 颜鹏; 张丽平; 韩文炎

doi:10.13305/j.cnki.jts.2018.04.003

茶叶科学 >

2018 , Vol. 38 >Issue 4: 353 - 362

DOI: https://doi.org/10.13305/j.cnki.jts.2018.04.003

外源水杨酸甲酯对高温胁迫下茶树光合作用和抗氧化酶的影响

魏吉鹏 ,
李鑫 ,
王朝阳 ,
李洋 ,
张兰 ,
沈晨 ,
颜鹏 ,
张丽平 ,
韩文炎

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1. 中国农业科学院茶叶研究所,浙江杭州 310008;
2. 安康市农业科学研究院,陕西安康 725000;
3. 河北农业大学园艺学院,河北保定 071000

魏吉鹏,男,硕士研究生,主要从事茶树栽培与生理生化研究。

收稿日期: 2017-07-31

修回日期: 2017-10-09

网络出版日期: 2019-10-15

基金资助

国家重点研发计划政府间国际科技创新合作重点专项（2017YFE0107500）、中国农业科学院科技创新工程（CAAS-ASTIP- 2014-TRICAAS）、中国农业科学院茶叶研究所基本科研业务费专项项目（1610212016025）

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Effects of Exogenous Salicylic Acid on Photosynthesis and Antioxidant Enzymes of Tea Plants under High Temperature

WEI Jipeng ,
LI Xin ,
WANG Zhaoyang ,
LI Yang ,
ZHANG Lan ,
SHEN Chen ,
YAN Peng ,
ZHANG Liping ,
HAN Wenyan

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1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Ankang Academy of Agricultural Sciences, Ankang 725000, China;
3. The Horticulture College of Hebei Agricultural University, Baoding 071000, China

Received date: 2017-07-31

Revised date: 2017-10-09

Online published: 2019-10-15

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摘要

近年来茶园高温灾害频发,而关于提高茶树耐热性的研究相对较少。本文以龙井43为试验材料,利用不同浓度水杨酸甲酯（MeSA）喷施茶苗后,在高温环境下（43℃）处理12βh,随后测定茶树叶片的净光合速率（Pn）,Rubisco最大羧化速率（V_c,_max）、RuBP最大再生速率（J_max）,电解质渗透率（EL）,丙二醛（MDA）含量以及抗氧化酶活性等相关指标。结果发现,1βmmol·L^-1 MeSA能够有效缓解高温导致的茶树Pn降低,维持V_c,_max和J_max稳定;高温导致茶树叶片EL和MDA含量迅速上升,而适当浓度的MeSA可显著降低高温环境下EL和MDA含量。此外,结果表明1βmmol·L^-1 MeSA能够提高APX和CAT活性,进而减少H₂O₂积累,减轻茶树细胞膜过氧化作用。综上所述,外源施用MeSA能够在一定程度上维持高温条件下植物叶片细胞光合系统稳定,提高茶树叶片的抗氧化酶活性,缓解氧化胁迫,最终提高茶树的耐热性。

关键词： 水杨酸甲酯; 茶树; 高温胁迫; 光合作用; 抗氧化酶

本文引用格式

魏吉鹏 , 李鑫 , 王朝阳 , 李洋 , 张兰 , 沈晨 , 颜鹏 , 张丽平 , 韩文炎 . 外源水杨酸甲酯对高温胁迫下茶树光合作用和抗氧化酶的影响[J]. 茶叶科学, 2018 , 38(4) : 353 -362 . DOI: 10.13305/j.cnki.jts.2018.04.003

Abstract

In recent years, heat stress was more frequently occurred in tea gardens. However, few studies were focused on the approaches towards the improvement of heat tolerance in tea plants. In this study, Longjing 43 was used as experimental material to investigate the effects of various concentrations of MeSA on the net photosynthetic rate (Pn), the maximum carboxylation rate of Rubisco (V_c_,max), the maximum RuBP regeneration rate (J_max), electrolyte leakage (EL), MDA content and antioxidant enzyme activities in tea leaves under heat stress. Results showed that 1βmmol·L^-1 MeSA could significantly increase Pn, V_c,_max and J_max in tea leaves under heat stress. EL and MDA content in tea leaves increased significantly after heat stress, while the application of MeSA attenuated heat-induced increases in EL and MDA. In addition, the application of 1βmmol·L^-1 MeSA stimulated the activities of APX and CAT, leading to an efficient scavenging of hydrogen peroxide (H₂O₂) in tea leaves. In summary, we revealed that the applications of MeSA could improve photosynthetic capacity, strengthen the antioxidant system, reduce reactive oxygen species accumulation and lipid peroxidation in tea leaves under heat stress, and thus improve the tolerance of tea plants under heat stress.

Key words： salicylic acid; tea plant; heat stress; photosynthesis; antioxidant enzyme

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