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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (5): 495-505.doi: 10.13305/j.cnki.jts.2019.05.001

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Cloning and Response Analysis of the CsMDHAR Gene Under the Abiotic Stress in Camellia sinensis

LIN Shijia, LI Hui, LIU Hao, TENG Ruimin, LIU Jingyu, WANG Shuang, ZHUANG Jing*   

  1. Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2019-05-08 Revised:2019-07-12 Online:2019-10-15 Published:2019-10-15

Abstract: In this study, a MDHAR gene (CsMDHAR) was cloned from Camellia sinensis cv. ‘Longjing 43’ based on the transcriptome data of tea plant. Sequence analysis shows that the open reading frame length of CsMDHAR was 1β305βbp, encoding 434 amino acids with a molecular weight of approximately 47.21βkDa and the theoretical isoelectric point of 5.99. CsMDHAR was a hydrophilic protein, including two unordered regions and 32 phosphorylation sites. CsMDHAR belonged to Pyr-redox-2 super-family containing a highly conserved region called FAD domain, and mainly composed of α-helix and random coil. PlantCARE and PLACE database prediction analysis suggest that there were many cis-elements related to light, hormones and stress resistance in the 1β000βbp upstream region of CsMDHAR gene. The expression profiles of CsMDHAR, CsAO and CsAPX in tea cv ‘Longjing 43’ and ‘Yingshuang’ under high temperature, low temperature, drought, and salt treatments were detected by qRT-PCR. The results indicate that the expression profiles of CsMDHAR, CsAO and CsAPX were suppressed under 4℃, and there were no significantly differences in ‘Longjing 43’ and ‘Yingshuang’. However, the expression profiles of CsMDHAR gene were upregulated under 38℃ or 200βg·L-1 PEG treatments in ‘Longjing 43’, with the highest 2.5 and 5 times of the control at 8βh and 2βh, respectively. In addition, the expression trends of CsAO and CsAPX in both cultivars were similar under NaCl (200βmmol·L-1) treatment, but the variation ranges were different, which might be related to the different stress response in tea plant.