研究了EGCG3′′Me、EGCG4′′Me和EGCG3′Me在正离子模式下的质谱裂解规律,并在此基础上分析了EGCG-O-甲基转移酶（EOMT）催化EGCG形成的反应产物。结果表明,EGCG-O-甲基转移酶催化EGCG后反应体系中生成了10余种不同的EGCG甲基化衍生物,主要包括EGCG3′′Me、EGCG4′′Me、EGCG3′Me、EGCG5′Me、EGCG3′′,4′′-diMe、EGCG3′′,5′′-diMe、EGCG3′,5′-diMe、EGCG3′,5′′-diMe、EGCG3′,3′′,5′′-triMe,以及EGCG5′,3′′,5′′-triMe等。
The fragmentation pathways of EGCG3′′Me, EGCG4′′Me and EGCG3′Me were studied using LC-MS under positive ionization mode, and the EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were analyzed. Results showed that more than ten kinds of EGCG methylated derivatives were identified, including EGCG3′′Me, EGCG4′′Me, EGCG3′Me, EGCG5′Me, EGCG3′′,4′′-diMe, EGCG3′′,5′′-diMe, EGCG3′,5′-diMe, EGCG3′,5′′-diMe, EGCG3′,3′′,5′′-triMe, EGCG5′,3′′,5′′-triMe.
[1] Mitsuaki Sano, Masazumi Suzuki, Toshio Miyase, et al. Novel anti-allergic catechin derivatives isolated from Oolong tea[J]. J Agric Food Chem, 1999, 47(5): 1906-1910.
[2] Maeda-Yamamoto M.Effects of tea infusions of varieties or different manufacturing types on inhibition of mouse mast cell activation[J]. Biosci Biotechnol Biochem, 1998, 62(11): 2277-2279.
[3] 吕海鹏, 谭俊峰, 林智. 茶树种质资源EGCG3"Me含量及其变化规律研究[J]. 茶叶科学, 2006, 26(4): 310-314.
[4] 吕海鹏, 林智, 谭俊峰, 等. 茶叶中EGCG3"Me的研究与开发[J]. 食品工业科技, 2008, 29(12): 275-277.
[5] 吕海鹏, 孙业良, 林智, 等. 表没食子儿茶素没食子酸酯的甲基化分子修饰[J]. 食品科学, 2010, 31(15): 139-142.
[6] 吕海鹏, 林智, 谭俊峰, 等. EGCG甲基化衍生物的清除DPPH自由基活性和总抗氧化活性[J]. 食品与发酵工业, 2011, 37(9): 166-170.
[7] 费冬梅, 林智, 吕海鹏, 等. EGCG-O-甲基转移酶(EOMT)在重组大肠杆菌中的表达条件研究[J]. 茶叶科学, 2011, 31(4): 333-340.
[8] 刘国强, 董静, 王弘, 等. EGC/GC和EGCG/GCG的ESI-IT-TOF质谱裂解规律研究[J]. 质谱学报, 2009, 30(5): 287-294.
[9] Miketova P, Schram K H, Whitney J, et al. Mass spectrometry of selected components of biological interest in green tea extracts[J]. Journal of Natural Products, 1998, 61(4): 461-467.
[10] 徐英, 董静, 王弘, 等. 电喷雾-离子阱-飞行时间质谱联用研究黄酮和异黄酮苷元C环上的裂解规律[J]. 高等学校化学学报, 2009, 30(1): 46-50.
[11] Okushio K, Suzuki M, Matsumoto N, et al. Methylation of tea catechins by rat liver homogenates[J]. Bioscience, Biotechnology, and Biochemistry, 1999, 63(2): 430-432.
[12] Hong L, Meng X F, Yang C S.Enzymology of methylation of tea catechins and inhibition of catechol-O-methyltransferase by (-)-epigallocatechin gallate[J]. The American Society for Pharmacology and Experimental Therapeutics, 2003, 31(5): 572-579.
[13] Zhu B T, Ushma K P, Cai X X.O-methylation of tea polyphenols catalyzed by human placental cytosolic catechol-O-methyltransferase[J]. The American Society for Pharmacology and Experimental Therapeutics, 2000, 28(9): 1024-1030.
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