以“紫娟”普洱茶为原料,分离制备茶褐素,采用膜技术对茶褐素分级,得到含量较多的3500Da透出部分和100000Da未透出部分,并对两部分进行理化性质分析。结果表明：100000Da未透出部分总酸性基团、羧基、酚羟基、多酚类及多糖含量均高于3500Da透出部分;100000Da未透出部分的多糖由核糖、鼠李糖、甘露糖、葡萄糖和半乳糖组成;而3500Da透出部分未检出单糖。研究显示,3500Da透出样和100000Da未透出部分中均含有-OH、-COOH,推测“紫娟”普洱茶茶褐素属苯多酚类物质,并含有酚性色素、多糖、蛋白质、脂类及生物碱等。
3500Da decomposed dialysate and 100000Da undecomposed dialysate of the theabrownin in ‘zijuan’ pu-erh tea as the main content were obtained by using membrane technology and their physicochemical properties were studied. The result showed that the total acidic functional group, carboxyl group, phenolic hydroxyl group,tea polyphenols, polysaccharose content of 100000Da undecomposed dialysate were higher than those of 3500Da decomposed dialysate. The polysaccharide isolated from 100000Da undecomposed dialysate was composed of ribose, rhamnose, mannose, glucose and galactose,but these monose were not detected in 3500Da decomposed dialysate. It suggested that the 3500Da decomposed dialysate and 100000Da undecomposed dialysate contained the -OH and -COOH groups. It was suggested that the theabrownin of the ‘zijuan’ pu-erh tea belonged to benzene polyphenolic substance and contained of polyphenolic pigments, lipids, polysaccharide, protein and alkaloids.
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