生长素相关基因在茶树腋芽冬季休眠不同生长阶段的表达分析

王新超; 马春雷; 杨亚军; 曹红利; 郝心愿; 金基强

doi:10.13305/j.cnki.jts.2012.06.012

茶叶科学 >

2012 , Vol. 32 >Issue 6: 509 - 516

DOI: https://doi.org/10.13305/j.cnki.jts.2012.06.012

生长素相关基因在茶树腋芽冬季休眠不同生长阶段的表达分析

王新超 ,
马春雷 ,
杨亚军 ,
曹红利 ,
郝心愿 ,
金基强

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中国农业科学院茶叶研究所,国家茶树改良中心,农业部茶树生物学与资源利用重点实验室,浙江杭州 310008

王新超（1975— ）,男,安徽霍邱人,博士,副研究员,主要从事茶树资源、育种与抗逆生物学研究。

收稿日期: 2012-05-30

修回日期: 2012-07-04

网络出版日期: 2019-09-05

基金资助

现代农业（茶叶）产业技术体系项目（CARS-23）、国家自然科学基金项目（30872059）资助

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Expression Analysis of Auxin-related Genes at Different Winter Dormant Stages of Axillary Buds in Tea Plant (Camellia sinensis)

WANG Xin-chao ,
MA Chun-lei ,
YANG Ya-jun ,
CAO Hong-li ,
HAO Xin-yuan ,
JIN Ji-qiang

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Tea Research Institute of Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China

Received date: 2012-05-30

Revised date: 2012-07-04

Online published: 2019-09-05

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摘要

利用实时定量PCR技术研究了从本实验室构建的茶树休眠芽与萌动芽抑制消减杂交文库中分离出来的与生长素相关的基因片段在茶树腋芽冬季休眠不同阶段的表达模式。研究结果表明：（1）与生长素响应因子同源的6条EST片段中,有5条的表达量休眠期高于萌发期,另外1条则相反。（2）与生长素原初反应基因同源的3条EST片段的表达模式存在差异,其中与SAURs同源的EST在休眠阶段上调表达,休眠解除以后下调表达。而与GH3和Aux/IAAs同源的2条EST在萌发阶段有一个表达高峰,而在休眠期和萌发以后,表达量都下降。（3）与生长素结合类蛋白同源的茶树CsGLP1表达量在萌发期高于休眠期。（4）与生长素内向运输载体基因同源的EST片段在深休眠期的表达量最高。（5）2条受生长素调控的EST表现出不同的表达模式,生长素诱导表达的EST在休眠期的表达量高于萌发期及随后阶段,而另外1条受生长素抑制的EST片段在萌发期的表达远远高于休眠期。这些结果初步说明生长素促进茶树腋芽的冬季休眠。

关键词： 茶树（Camellia sinensis）; 芽休眠与萌发; 生长素相关基因; 表达分析

本文引用格式

王新超 , 马春雷 , 杨亚军 , 曹红利 , 郝心愿 , 金基强 . 生长素相关基因在茶树腋芽冬季休眠不同生长阶段的表达分析[J]. 茶叶科学, 2012 , 32(6) : 509 -516 . DOI: 10.13305/j.cnki.jts.2012.06.012

Abstract

The expression patterns of some auxin-related genes, including auxin response factors (ARFs), primary auxin responsive genes, auxin-regulated genes, auxin influx carrier gene and auxin-binding protein (ABP) gene, were studied in different stages of tea plant axillary bud dormancy and its release. The results showed that (1) the expression levels of five in six ARF homologous EST fragments were up-regulated in the deep dormancy and down-regulated after sprouted, the level of the last one showed the opposite expression levels. (2) There were significant differences among the expression patterns of 3 ESTs which are homologous with primary auxin responsive genes. EST which is homologous with SAURs was up-regulated in the dormancy stage but down-regulated after dormancy release. On the contrary, the 2 ESTs which are homologous with GH3 and Aux/IAAs were up-regulated in sprouting stage while down-regulated in dormant stages and after sprouting stages. (3) The CsGLP1 gene which was homogenous with ABPs has a lower expression level in the dormancy stage but maintain rising trend after germination.(4) The EST which is homogenous with auxin influx carrier gene showed the highest expression levels during the deep dormant stage but maintain lower levels in other stages. (5) Two auxin-regulated ESTs showed different expression patterns. The expression levels of auxin-induced EST were higher in the dormant stages than those in the sprouting stages, while the levels of the other auxin-repressed EST were higher in the sprouting stage than that in the dormant stages. These results indicated that auxin promote the dormancy of tea axillary buds in winter.

Key words： tea plant (Camellia sinensis); bud dormancy and budbreak; auxin-related gene; expression analysis

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