依据保健食品检验与评价技术规范对茶氨酸复合制剂的缓解体力疲劳功能进行评价。人体体质量以60kg计,样品人体推荐剂量为每日1.4g,采用昆明种小鼠设定低、中、高3个剂量组分别为0.12、0.23、0.70g/kg,分别相当于人体推荐剂量的5、10、30倍灌胃,对照组以等量蒸馏水灌胃,连续30d。结果显示,高剂量组能明显延长小鼠负重游泳时间,降低血清尿素含量,增加肝糖原含量,并对小鼠运动前后血乳酸含量无影响,表明茶氨酸复合制剂具有缓解体力疲劳的功能。
According to “The Standard of Health Food Test and Assesment”, the alleviating physical fatigue function of theanine compound preparation was evaluated. The recommended daily dose of human is 1.4g/60kg body weight. The low, middle and high dosage groups of Kunming mice were respectively 0.12, 0.23 and 0.70g/kg, equivalent to 5, 10 and 30 times of human recommended doses. The control group with equal distilled water by gavage, on 30 consecutive days. The results showed that 0.70g/kg dose group could significantly prolong the loaded swimming time of mice, reduce the serum urea content and increase hepatic glycogen content, indicating the theanine compound preparation possessed the function of alleviating physical fatigue.
[1] 刘显明, 李月芬, 李国平. 茶氨酸对D-半乳糖衰老模型小鼠抗衰老作用的实验研究[J]. 创伤外科杂志, 2008, 10(3): 257-259.
[2] 小林加奈理. L-テアニンのヒトの脑波に及ぼす影响[J]. 日本农芸化学会志, 1998, 72: l53-l57.
[3] BUKOWSKI J F, PERCIVAL S S.L-theanine intervention enhances human gamma delta T lymphocyte function[J]. Nutrition reviews, 2008, 66(2): 96-102.
[4] KIMURA K, OZEKI M, JUNEJA L R, et al. L-theanine reduces psychological and physiological stress responses[J]. Biological Psychology, 2007, 4: 39-45.
[5] KAMATH A B, WANG L, DAs H, et al. Antigens in tea-beverage prime human Vγ2Vδ2 T cell in vitro and in vivo for memory and nonmemory antibacterial cytokine responses[J]. Proceedings of the National Academy of Sciences, 2003, 100: 6009-6014.
[6] 林雪玲, 程朝辉, 黄才欢, 等. 茶氨酸对小鼠学习记忆能力的影响[J]. 食品科学, 2004, 25(5): 171-173.
[7] 王小雪, 邱隽, 宋宇, 等. 茶氨酸的抗疲劳作用研究[J]. 中国公共卫生, 2002, 18(3): 315-317.
[8] 李敏, 沈新南, 姚国英. 茶氨酸延缓运动性疲劳及其作用机制研究[J]. 营养学报, 2005, 27(4): 326-329.
[9] 李靓, 林智, 何普明, 等. 茶氨酸改善小鼠睡眠状况的实验研究[J]. 食品科学, 2009, 30(15): 214-216.
[10] 中华人民共和国卫生部. 保健食品检验与评价技术规范实施手册: 2003年版[M]. 北京: 中华人民共和国卫生部, 2003: 756-761.
[11] 谭俊峰, 林智, 李靓. 茶氨酸复合制剂安全性毒理学研究[J]. 食品科学, 2011, 32(19): 262-267.
浙公网安备 33019902000101号 
