茶叶样品经甲醇提取,二氯甲烷反萃取,再经ENVI-18小柱净化,采用液相色谱,紫外检测器检测,测定茶叶中吡虫啉农药的残留量。茶叶中吡虫啉的添加浓度为0.1、0.5、2.5 mg/kg,其回收率范围81.5%~87.9%,相对标准偏差（RSD）≤10.2%,方法最低检出浓度为0.05 mg/kg,符合农药残留分析要求。
A method was developed for determination of imidacloprid residue in tea by high performance liquid chromatography (HPLC) with UV detector. Imidacloprid in tea samples was extracted with methyl alcohol and re-extracted by dichloromethane then cleaned up with the ENVI-18 cartridge. Imdacloprid was eluted with acetonitrile:water(v/v, 30:70) solution from the cartridge and detected with HPLC. The recovery of imidacolprid ranged from 81.5%~87.9% with the RSD less than 10.2%. Practical determination limit was 0.05 mg/kg.
[1] 张一宾, 张怿. 世界农药新进展[M]. 北京: 化学工业出版社, 2007.
[2] 汤富彬, 刘光明, 罗逢健, 等. 茶叶中吡虫啉残留量的HPLC测定方法[J]. 农药, 2004, 43(12): 561~562.
[3] 吴刚, 吴云华, 鲍晓霞, 等. 高效液相色谱法分析茶叶中吡虫啉残留量[J]. 理化分析-化学分册, 2005, 41(11): 768~769.
[4] Fernandez-Alba A R. Determination of imidacloprid invegetables by high performance liquid chromatography with diode-array detection[J]. Journal of Chromatography A, 1996, 721: 97~105.
[5] Navalon A, Gonzalez-Casado A, El-Khattabi R, et al. Determination of imidacloprid invegetable samples by gas chromatography-mass spectrometry[J]. Analyst, 1997, 122: 579~581.
[6] M Rancan, A G Sabatini, G Achilli, ,et al. Determination of Imidacloprid. Determination of Imidacloprid and metabolites by liquid chromatography with an electrochemical detector and post column photochemical reactor. [J]. Analytica Chimica Acta, 2006, 555, (1): 20~24.
[7] J M Bonmatin, P A Marchand, R Charvet, et al. Quantification of Imidacloprid Uptake in Maize Crops[J]. J Agric Food Chem, 2005, 53: 5336~5341.
[8] Totti S, Fernandez M, Ghini S, et al. Application of matrix solid phase dispersion to the determination of imidacloprid, carbaryl, aldicarb, and their main metabolites in honeybees by liquid chromatography-mass spectrometry detection[J]. Talanta, 2006, 69: 724~729.
[9] Monika Gupta, Anupam Sharma, Adarsh Shanker.Dissipation of imidacloprid in Orthodox tea and its transfer from made tea to infusion[J]. Food Chemistry, 2008, 106: 158~1640.
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