采用制备HPLC系统进行分离纯化并进一步结合NMR、LC/MS进行结构鉴定,从茶叶中分离纯化出了EGCG3″Me;研究了不同提取条件对茶叶中EGCG3″Me含量的影响,初步建立了用反相高效液相色谱测定茶叶中EGCG3″Me含量的分析方法。结果表明,该方法重现性好,适用于常规定量分析,平均加样回收率为95.48%,RSD为3.31%;采用蒸馏水在90℃的水浴浸提5 min,茶汤中EGCG3"Me的浓度可以达到最高值。
EGCG3″Me was isolated by preparative HPLC system from tea and identified by NMR and LC/MS. The influence of different extracting method on the EGCG3″Me content in tea was discussed, and a simple high performance liquid chromatography analytical method of EGCG3″Me was developed. Results showed that the analytical method showed a good linear relationship between the peak area and content of EGCG3″Me (R2=0.9998) and high reproducibility. The average recovery rate of EGCG3″Me was 95.48% and the relative standard deviation(RSD)was 3.31%. The EGCG3″Me concentration in tea infusion can reach highest when the tea sample was immersed in distilled water for 5 min at 90℃.
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