采用气相色谱（GC）对茶籽的脂肪酸组成进行分析,并研究了活性脂肪酸对高浓度葡萄糖胁迫下RF/6A细胞生长的影响及其与葡萄糖对RF/6A细胞脂肪酸代谢的调节。结果表明,油酸与亚油酸为茶籽中含量最高的脂肪酸;而茶籽中脂肪酸的组成与其品种和产地有密切关系。亚油酸与亚麻酸对高浓度葡萄糖诱导的RF/6A细胞过度增殖有抑制作用。亚油酸、亚麻酸及棕榈酸对RF/6A细胞的脂肪酸代谢均具有调节作用。
Fatty acids in tea seeds have been analyzed by gas chromatography. The effects of the bioactive fatty acids in tea seeds on the cytotoxicity induced by high concentration of glucose in RF/6A cells have been investigated, and regulation of fatty acids and glucose on the metabolism of fatty acids in RF/6A cells have been primarily assessed at the same time. The results showed that oleic acid and linoleic acid are the main components of the fatty acids in tea seeds. Their compositions were closely related with their varieties and productive location. Both, the linoleic acid and linolenic acid suppress the excessive proliferation of RF/6A cells induced by high concentrations of glucose; linoleic acid, linolenic acid and palmitic acid also affect the metabolism of fat.
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