茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响

doi:10.13305/j.cnki.jts.2009.6.03

茶叶科学 ›› 2009, Vol. 29 ›› Issue (6): 419-425.doi: 10.13305/j.cnki.jts.2009.6.03

茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响

于海宁^1,2, 单伟光¹, Undurti N DAS³, 沈生荣^2,*

1. 浙江工业大学药学院,浙江杭州 310014;
2. 浙江大学食品科学与营养学系,浙江杭州 310029;
3. UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH 44120, USA

收稿日期:2008-12-22 修回日期:2009-08-13 发布日期:2019-09-09
通讯作者: * , Email: shrshen@zju.edu.cn
作者简介:于海宁（1977— ）,女,博士,副教授,主要从事天然药物的研究与开发。
基金资助:
本课题为美国UND Life Sciences合作项目的一部分

Compositions of Fatty Acids in Tea Seeds and Effects of Their Bioactive Fatty Acids on Glucose-induced Cytotoxicity in RF/6A Cells

YU Hai-ning^1,2, SHAN Wei-guang¹, Undurti N DAS³, SHEN Sheng-rong^2,*

1. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China;
2. Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China;
3. UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH 44120, USA

Received:2008-12-22 Revised:2009-08-13 Published:2019-09-09

摘要/Abstract

摘要： 采用气相色谱（GC）对茶籽的脂肪酸组成进行分析,并研究了活性脂肪酸对高浓度葡萄糖胁迫下RF/6A细胞生长的影响及其与葡萄糖对RF/6A细胞脂肪酸代谢的调节。结果表明,油酸与亚油酸为茶籽中含量最高的脂肪酸;而茶籽中脂肪酸的组成与其品种和产地有密切关系。亚油酸与亚麻酸对高浓度葡萄糖诱导的RF/6A细胞过度增殖有抑制作用。亚油酸、亚麻酸及棕榈酸对RF/6A细胞的脂肪酸代谢均具有调节作用。

关键词: 茶籽, 脂肪酸, RF/6A细胞, 高糖胁迫

Abstract: Fatty acids in tea seeds have been analyzed by gas chromatography. The effects of the bioactive fatty acids in tea seeds on the cytotoxicity induced by high concentration of glucose in RF/6A cells have been investigated, and regulation of fatty acids and glucose on the metabolism of fatty acids in RF/6A cells have been primarily assessed at the same time. The results showed that oleic acid and linoleic acid are the main components of the fatty acids in tea seeds. Their compositions were closely related with their varieties and productive location. Both, the linoleic acid and linolenic acid suppress the excessive proliferation of RF/6A cells induced by high concentrations of glucose; linoleic acid, linolenic acid and palmitic acid also affect the metabolism of fat.

Key words: tea (Camellia sinensis) seed, fatty acids, RF/6A cells, high glucose-induced

中图分类号:

于海宁, 单伟光, Undurti N DAS, 沈生荣. 茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响[J]. 茶叶科学, 2009, 29(6): 419-425. doi: 10.13305/j.cnki.jts.2009.6.03.

YU Hai-ning, SHAN Wei-guang, Undurti N DAS, SHEN Sheng-rong. Compositions of Fatty Acids in Tea Seeds and Effects of Their Bioactive Fatty Acids on Glucose-induced Cytotoxicity in RF/6A Cells[J]. Journal of Tea Science, 2009, 29(6): 419-425. doi: 10.13305/j.cnki.jts.2009.6.03.

参考文献

[1] 陈椽. 茶药学[M]. 北京: 中国展望出版社, 1987.
[2] Lee CP, Shih PH, Hsu CL, et al. Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl₄-induced oxidative damage in rats[J]. Food and Chemical Toxicology, 2007, 45: 888~895.
[3] Lee CP, Yen GC.Antioxidant activity and bioactive compounds of tea seed (Camellia oleifera Abel.) oil[J]. Journal of Agricultural and Food Chemistry, 2006, 54: 779~784.
[4] Yoshikawa M, Morikawa T, Nakamura S, et al. Bioactive saponins and glycosides. XXV. Acylated oleanane-type triterpene saponins from the seeds of tea plant (Camellia sinensis)[J]. Chemical & Pharmaceutical Bulletin, 2007, 55: 57~63.
[5] Morikawa T, Li N, Nagatomo A, et al. Triterpene saponins with gastroprotective effects from tea seed (the seeds of Camellia sinensis)[J]. Journal of Natural Products, 2006, 69: 185~190.
[6] Hayashi K, Sagesaka YM, Suzuki T, et al. Inactivation of human type A and B influenza viruses by tea-seed saponins[J]. Bioscience Biotechnology and Biochemistry, 2000, 64: 184~186.
[7] Connor KM, SanGiovanni JP, Lofqvist C, et al. Increased dietary intake of omega-3-polyunsaturated fatty acids reduces pathological retinal angiogenesis[J]. Nature Medicine, 2000, 13: 868~873.
[8] Heinemann KM, Waldron MK, Bigley KE, et al. Long-chain (n-3) polyunsaturated fatty acids are more efficient than alpha-linolenic acid in improving electroretinogram responses of puppies exposed during gestation, lactation, and weaning[J]. The Journal of Nutrition, 2005, 135: 1960~1966.
[9] Shen SR, Yu HN, Chen P, et al. Fatty acids in tea shoots (Camellia sinensis (L.) O. Kuntze) and their effects on the growth of retinal RF/6A endothelial cell lines[J]. Molecular Nutrition & Food Research, 2007, 51: 221~228.
[10] Udell T, Gibson RA, Makrides M et al. The effect of alpha-linolenic acid and linoleic acid on the growth and development of formula-fed infants: a systematic review and meta-analysis of randomized controlled trials[J]. Lipids, 2005, 40: 1~11.
[11] Myśliwiec M, Balcerska A, Zorena K, et al. The role of vascular endothelial growth factor, tumor necrosis factor alpha and interleukin-6 in pathogenesis of diabetic retinopathy[J]. Diabetes Research and Clinical Practice, 2008, 79: 141~146.
[12] Dills DG, Moss SE, Klein R et al. Association of elevated IGF-I levels with increased retinopathy in late-onset diabetes[J]. Diabetes, 1991, 40: 1725~1730.
[13] Henricsson M, Berntorp K, Berntorp E, et al. (1999) Progression of retinopathy after improved metabolic control in type 2 diabetic patients. Relation to IGF-1 and hemostatic variables[J]. Diabetes Care, 1999, 22: 1944~1949.
[14] Chen W, Jump DB, Esselman WJ, et al. Inhibition of cytokine signaling in human retinal endothelial cells through modification of caveolae/lipid rafts by docosahexaenoic acid[J]. Investigative Ophthalmology & Visual Science, 2007, 48: 18~26.
[15] Kulacoglu DN, Kocer I, Kurtul N, et al. Alterations of fatty acid composition of erythrocyte membrane in type 2 diabetes patients with diabetic retinopathy[J]. Japanese Journal of Ophthalmology, 2003, 47: 551~556.
[16] Hodge AM, English DR, O'Dea K, et al. Plasma phospholipid and dietary fatty acids as predictors of type 2 diabetes: interpreting the role of linoleic acid[J]. The American Journal of Clinical Nutrition, 2007, 86: 189~197.
[17] Lu M, Taylor A, Chylack LT Jr, et al. Dietary fat intake and early age-related lens opacities[J]. The American Journal of Clinical Nutrition, 2005, 81: 773~779.
[18] Trimborn M, Iwig M, Glanz D, et al. Linoleic acid cytotoxicity to bovine lens epithelial cells: influence of albumin on linoleic acid uptake and cytotoxicity[J]. Ophthalmic Research, 2000, 32: 87~93.
[19] Burdge GC, Sala-Vila A, West AL, et al. The effect of altering the 20:5n-3 and 22:6n-3 content of a meal on the postprandial incorporation of n-3 polyunsaturated fatty acids into plasma triacylglycerol and non-esterified fatty acids in humans[J]. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 2007, 77: 59~65.

茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响

Compositions of Fatty Acids in Tea Seeds and Effects of Their Bioactive Fatty Acids on Glucose-induced Cytotoxicity in RF/6A Cells

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	孙颖, 陈鑫, 杨华, 应剑, 邵丹青, 吕晓华, 肖杰, 陈志雄, 李颂, 覃俊杰, 郑斌, 高建设. 饮用金花香橼茶3个月对小样本高脂血症人群糖脂代谢的改善效果研究[J]. 茶叶科学, 2022, 42(4): 561-576.
[2]	向晶, 梁月荣, 赵东, 王开荣, 陆建良, 袁名安, 郑新强. 不同品种、地区茶叶籽仁含油量及茶叶籽油中脂肪酸组分和含量差异性分析[J]. 茶叶科学, 2022, 42(2): 233-248.
[3]	吴万富, 吕世懂, 杨学芳, 李悦, 张继光. 云南大叶种茶树籽油的特征指标与脂肪酸组成研究[J]. 茶叶科学, 2021, 41(4): 463-470.
[4]	陈明杰, 杜正花, 秦健恒, 李若钰, 于朝夕, 郭丽. 不同种质茶籽脂质代谢特征分析[J]. 茶叶科学, 2021, 41(3): 350-360.
[5]	常亚丽, 黄晓兵, 蒋双丰, 黄双杰, 孙慕芳, 刘威, 郭桂义. 豫南茶树种质资源籽实脂肪含量及脂肪酸组成分析[J]. 茶叶科学, 2020, 40(3): 352-362.
[6]	郭丽, 吕海鹏, 陈明杰, 张悦, 把熠晨, 郭雅玲, 林智. 福建乌龙茶脂肪酸含量及差异性分析[J]. 茶叶科学, 2019, 39(5): 611-618.
[7]	侯爱香, 颜道民, 孙静文, 郑旭, 李赛丹, 肖润花, 贝爽. 绿茶、红茶和茯砖茶水提物对肠道微生物体外发酵特性的影响[J]. 茶叶科学, 2019, 39(4): 403-414.
[8]	叶颖, 韦保耀, 滕建文, 夏宁, 黄丽, 曾司斌. 六堡茶对高脂饮食大鼠肠道短链脂肪酸含量的影响[J]. 茶叶科学, 2019, 39(2): 211-219.
[9]	陈丹, 俞滢, 岳川, 王鹏杰, 陈静, 陈桂信, 叶乃兴. 茶树△12-脂肪酸去饱和酶基因FAD2和FAD6的克隆与表达分析[J]. 茶叶科学, 2017, 37(6): 541-550.
[10]	龚志华, 梁丹, 张志博, 肖文军. 微波辅助提取茶籽皂苷的研究[J]. 茶叶科学, 2013, 33(4): 358-363.
[11]	郑德勇, 常玉玺, 叶乃兴, 杨江帆. 茶籽成熟过程中主要功能成分变化规律研究[J]. 茶叶科学, 2013, 33(3): 253-260.
[12]	常玉玺, 郑德勇, 叶乃兴, 杨江帆. 福建茶树种质资源的茶籽油脂肪酸组成分析[J]. 茶叶科学, 2012, 32(1): 22-28.
[13]	彭忠瑾, 麻成金, 肖旺, 黄婷, 郑君晓, 向勇平. 茶籽油制备生物柴油工艺研究[J]. 茶叶科学, 2012, 32(1): 44-52.
[14]	郑生宏, 李大祥, 方世辉, 汤志近. 茶籽壳酸水解制备木糖工艺研究[J]. 茶叶科学, 2011, 31(3): 195-200.
[15]	董瑞霞, 李立祥, 蒋其忠. 茶籽壳原花青素的鉴定与分析[J]. 茶叶科学, 2011, 31(2): 119-123.