茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响

于海宁; 单伟光; Undurti N DAS; 沈生荣

doi:10.13305/j.cnki.jts.2009.6.03

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茶叶科学 ›› 2009, Vol. 29 ›› Issue (6) : 419-425. DOI: 10.13305/j.cnki.jts.2009.6.03

茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响

于海宁^1,2, 单伟光¹, Undurti N DAS³, 沈生荣^2,*

作者信息 +

Compositions of Fatty Acids in Tea Seeds and Effects of Their Bioactive Fatty Acids on Glucose-induced Cytotoxicity in RF/6A Cells

YU Hai-ning^1,2, SHAN Wei-guang¹, Undurti N DAS³, SHEN Sheng-rong^2,*

Author information +

文章历史 +

摘要

采用气相色谱（GC）对茶籽的脂肪酸组成进行分析,并研究了活性脂肪酸对高浓度葡萄糖胁迫下RF/6A细胞生长的影响及其与葡萄糖对RF/6A细胞脂肪酸代谢的调节。结果表明,油酸与亚油酸为茶籽中含量最高的脂肪酸;而茶籽中脂肪酸的组成与其品种和产地有密切关系。亚油酸与亚麻酸对高浓度葡萄糖诱导的RF/6A细胞过度增殖有抑制作用。亚油酸、亚麻酸及棕榈酸对RF/6A细胞的脂肪酸代谢均具有调节作用。

Abstract

Fatty acids in tea seeds have been analyzed by gas chromatography. The effects of the bioactive fatty acids in tea seeds on the cytotoxicity induced by high concentration of glucose in RF/6A cells have been investigated, and regulation of fatty acids and glucose on the metabolism of fatty acids in RF/6A cells have been primarily assessed at the same time. The results showed that oleic acid and linoleic acid are the main components of the fatty acids in tea seeds. Their compositions were closely related with their varieties and productive location. Both, the linoleic acid and linolenic acid suppress the excessive proliferation of RF/6A cells induced by high concentrations of glucose; linoleic acid, linolenic acid and palmitic acid also affect the metabolism of fat.

导出引用

于海宁, 单伟光, Undurti N DAS, 沈生荣. 茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响[J]. 茶叶科学. 2009, 29(6): 419-425 https://doi.org/10.13305/j.cnki.jts.2009.6.03

YU Hai-ning, SHAN Wei-guang, Undurti N DAS, SHEN Sheng-rong. Compositions of Fatty Acids in Tea Seeds and Effects of Their Bioactive Fatty Acids on Glucose-induced Cytotoxicity in RF/6A Cells[J]. Journal of Tea Science. 2009, 29(6): 419-425 https://doi.org/10.13305/j.cnki.jts.2009.6.03

中图分类号： TS272 Q946.81

参考文献

[1] 陈椽. 茶药学[M]. 北京: 中国展望出版社, 1987.
[2] Lee CP, Shih PH, Hsu CL, et al. Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl₄-induced oxidative damage in rats[J]. Food and Chemical Toxicology, 2007, 45: 888~895.
[3] Lee CP, Yen GC.Antioxidant activity and bioactive compounds of tea seed (Camellia oleifera Abel.) oil[J]. Journal of Agricultural and Food Chemistry, 2006, 54: 779~784.
[4] Yoshikawa M, Morikawa T, Nakamura S, et al. Bioactive saponins and glycosides. XXV. Acylated oleanane-type triterpene saponins from the seeds of tea plant (Camellia sinensis)[J]. Chemical & Pharmaceutical Bulletin, 2007, 55: 57~63.
[5] Morikawa T, Li N, Nagatomo A, et al. Triterpene saponins with gastroprotective effects from tea seed (the seeds of Camellia sinensis)[J]. Journal of Natural Products, 2006, 69: 185~190.
[6] Hayashi K, Sagesaka YM, Suzuki T, et al. Inactivation of human type A and B influenza viruses by tea-seed saponins[J]. Bioscience Biotechnology and Biochemistry, 2000, 64: 184~186.
[7] Connor KM, SanGiovanni JP, Lofqvist C, et al. Increased dietary intake of omega-3-polyunsaturated fatty acids reduces pathological retinal angiogenesis[J]. Nature Medicine, 2000, 13: 868~873.
[8] Heinemann KM, Waldron MK, Bigley KE, et al. Long-chain (n-3) polyunsaturated fatty acids are more efficient than alpha-linolenic acid in improving electroretinogram responses of puppies exposed during gestation, lactation, and weaning[J]. The Journal of Nutrition, 2005, 135: 1960~1966.
[9] Shen SR, Yu HN, Chen P, et al. Fatty acids in tea shoots (Camellia sinensis (L.) O. Kuntze) and their effects on the growth of retinal RF/6A endothelial cell lines[J]. Molecular Nutrition & Food Research, 2007, 51: 221~228.
[10] Udell T, Gibson RA, Makrides M et al. The effect of alpha-linolenic acid and linoleic acid on the growth and development of formula-fed infants: a systematic review and meta-analysis of randomized controlled trials[J]. Lipids, 2005, 40: 1~11.
[11] Myśliwiec M, Balcerska A, Zorena K, et al. The role of vascular endothelial growth factor, tumor necrosis factor alpha and interleukin-6 in pathogenesis of diabetic retinopathy[J]. Diabetes Research and Clinical Practice, 2008, 79: 141~146.
[12] Dills DG, Moss SE, Klein R et al. Association of elevated IGF-I levels with increased retinopathy in late-onset diabetes[J]. Diabetes, 1991, 40: 1725~1730.
[13] Henricsson M, Berntorp K, Berntorp E, et al. (1999) Progression of retinopathy after improved metabolic control in type 2 diabetic patients. Relation to IGF-1 and hemostatic variables[J]. Diabetes Care, 1999, 22: 1944~1949.
[14] Chen W, Jump DB, Esselman WJ, et al. Inhibition of cytokine signaling in human retinal endothelial cells through modification of caveolae/lipid rafts by docosahexaenoic acid[J]. Investigative Ophthalmology & Visual Science, 2007, 48: 18~26.
[15] Kulacoglu DN, Kocer I, Kurtul N, et al. Alterations of fatty acid composition of erythrocyte membrane in type 2 diabetes patients with diabetic retinopathy[J]. Japanese Journal of Ophthalmology, 2003, 47: 551~556.
[16] Hodge AM, English DR, O'Dea K, et al. Plasma phospholipid and dietary fatty acids as predictors of type 2 diabetes: interpreting the role of linoleic acid[J]. The American Journal of Clinical Nutrition, 2007, 86: 189~197.
[17] Lu M, Taylor A, Chylack LT Jr, et al. Dietary fat intake and early age-related lens opacities[J]. The American Journal of Clinical Nutrition, 2005, 81: 773~779.
[18] Trimborn M, Iwig M, Glanz D, et al. Linoleic acid cytotoxicity to bovine lens epithelial cells: influence of albumin on linoleic acid uptake and cytotoxicity[J]. Ophthalmic Research, 2000, 32: 87~93.
[19] Burdge GC, Sala-Vila A, West AL, et al. The effect of altering the 20:5n-3 and 22:6n-3 content of a meal on the postprandial incorporation of n-3 polyunsaturated fatty acids into plasma triacylglycerol and non-esterified fatty acids in humans[J]. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 2007, 77: 59~65.