采用平板稀释法以孟加拉红（DRBC）和18%的甘油琼脂（DG18）两种培养基对60个普洱茶样品中的真菌种群进行了分离,种群分离结果显示：样品中真菌种群数量变化值范围为1.6×103~1.16×105 cfu/g;应用分子生物学的手段,对分离到的71株真菌做了分子鉴定,鉴定结果表明：这些真菌分别属于19个属,40个种,主要是曲霉属13种,青霉属7种,其他属20种。数量上占优势的真菌种群是酵母和曲霉属的不同种。这些真菌的安全性有待进一步评估。
The fungal colonization was investigated from 60 representative samples by using the method of dilution plating on the medium of Dichloran Rose-Bengal Chloramphenicol Agar (DRBC) and dichloran 18% glycerol (DG18) agar. The results of the survey showed that the fungal colonization ranged from 1.6×103 to 1.16×105 cfu/g, and 71 isolates were identified by means of sequencing the rDNA fragments, which belong to 40 species of 19 genera, including 13 species of Aspergillus and 7 species of Penicillium, and 20 species of other genera. The predominant fungi in number were yeasts and different species of the genus Aspergillus. Further evaluation on the safety of these fungi isolated from Pu-erh tea products is needed.
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