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毛细管电泳分析茶黄素的方法研究

  • 夏文娟 ,
  • 张丽霞 ,
  • 史作安 ,
  • 贾明
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  • 山东农业大学园艺科学与工程学院,山东 泰安 271018
夏文娟(1980-),山东潍坊人,硕士研究生,研究方向:茶叶生物化学。

收稿日期: 2005-09-26

  修回日期: 2006-03-01

  网络出版日期: 2019-09-10

基金资助

山东省教育厅重点项目(J02H04)

Analysis of Theaflavins by Capillary Electrophoresis

  • XIA Wen-juan ,
  • ZHANG Li-xia ,
  • SHI Zuo-an ,
  • JIA Ming
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  • College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China

Received date: 2005-09-26

  Revised date: 2006-03-01

  Online published: 2019-09-10

摘要

研究了毛细管电泳分析茶黄素类组分的最佳条件。结果表明:在250βmmol/L H3BO4、10βmmol/L KH2PO4、0.75βmmol/L SDS和20%(v/v)乙腈组成的pH7.5电泳介质中,选择25βkV电压、20℃柱温和200βnm波长检测,可在10βmin以内将茶叶中的四种主要茶黄素单体分离,并获得较高的检测灵敏度。四种茶黄素单体浓度与峰面积值之间的相关系数r=0.9945~0.9990,检测下限在0.53~0.64βμg/ml之间,加标回收率为92.50%~108.36%,变异系数≤2.67%。本方法快速、准确,可用于实际样品的测定。

本文引用格式

夏文娟 , 张丽霞 , 史作安 , 贾明 . 毛细管电泳分析茶黄素的方法研究[J]. 茶叶科学, 2006 , 26(2) : 147 -153 . DOI: 10.13305/j.cnki.jts.2006.02.014

Abstract

The investigation optimum analytical conditions in the determination of theaflavins(TFs) with capillary electrophoresis was described. The electrolyte solution was consisted of 250βmmol/L H3BO4, 10βmmol/L KH2PO4, 0.75βmmol/L SDS and 20% (v/v) acetonitrile under pH7.5. The work parameters of the instrument were selected as follows: voltage 25βkV, column temperature 20℃, and detecting wavelength 200βnm. By using this method, the four majors of TF could be separated each other completely within 10 minutes and each had a good linear relationship between its peak area and corresponding concentration (r=0.9945~0.9990). The minimum concentration limit was between 0.53~0.64βμg/ml. The mean recoveries of TFs monomers varied from 92.50% to 108.36%. The coefficients of variation were less than 2.67%. Results showed that the method was simple, rapid and accurate for TFs to be determined in black tea and TFs products.

参考文献

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