采用HPLC分析技术对国内茶树种质资源的EGCG3"Me含量及其变化规律进行了研究。结果表明：在200份高多酚茶树种质中发现EGCG3"Me的含量大于1%的茶树种质有6个;茶梢中EGCG3"Me的含量随着叶片成熟度的提高而增加,并且秋季新梢高于夏季新梢;绿茶加工过程能有效保持鲜叶中EGCG3"Me含量,而乌龙茶加工过程的萎凋工序中茶叶中EGCG3"Me含量有明显增加。
The changes of EGCG3"Me content in different tea germplasms, crop seasons and tea processing were studied by HPLC. The content of EGCG3"Me in six tested tea cultivars was more than 1% among 200 tea germplasms containing high tea polyphenols. The content of EGCG3"Me in new tea shoot was increased with the leaf maturity, and was higher in autumn tea than in summer tea. The content of EGCG3"Me was not changed obviously during the process of green tea manufacture, but increased distinctly during the withering process of the Oolong tea.
[1] Mitsuaki Sano, Masazumi Suzuki, Toshio Miyase, et al. Novel anti-allergic catechin derivatives isolated from oolong tea[J]. J Agric Food Chem, 1999, 47(5): 1906~1910.
[2] Maeda-Yamamoto M.Effects of tea infusions of varieties or different manufacturing types on inhibition of mouse mast cell activation[J]. Biosci.Biotechnol.Biochem, 1998, 62(11): 2277~2279.
[3] Mari Maeda-Yamamoto, Naoki Inagaki, Jiro Kitaura, et al. O-Methylated Catechins from Tea Leaves Inhibit Mutiple Protein Kinases in Mast Cells[J]. The Journal of Immunology, 2004, 172: 4486~4492.
[4] FENG-LAN Chiu, JEN-KUN LIN.HPLC Analysis of Naturally Occurring Methylated Catechins,3’’- and 4’’-Methyl-epigallocatechin Gallate, in Various Fresh Tea Leaves and Commercial Teas and Their Potent Inhibitory Effects on Inducible Nitric Oxide Synthase in Macrophages[J]. JAgric Food Chem, 2005, 53: 7035~7042.
[5] Sano M.Inhibitory effects of O-methylated catechin derivatives on mouse type-I allergy. Proceedings of the 2001 International Conference on O-CHA(tea) Culture and Science, Session Ⅲ, Health and Benefits[C]. Shizuoka, Japan, October 5~8, 2001: 159~162.
[6] Maeda-Yamamoto, M. Anti-allegic and anti-cancer metastatic actions of green tea[J]. Nippon Shokuhin kagaku Kaishi, 2002, 49(10): 631~638.
[7] Maeda-Yamamoto, M. The change of Epigallocatechin-3-o-(3-o-methyl) gallate content in tea of different varieties, tea seasons of crop and processing method[J]. Nippon Shokuhin Kagaku Kaishi, 2001, 1(48): 64~68.
[8] KYOJI YOSHINO, KENJIRO OGAWA, TOSHIO MIYASE, et al. Inhibitory Effects of the C-2 Epimeric Isomers of Tea Catechins on Mouse Type IV Allergy[J]. J Agric Food Chem, 2004, 52(15): 4660~4663.
浙公网安备 33019902000101号 
