建立了高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定茶叶中单糖和双糖的检测方法。采用Shodex NH₂P-50-4E（4.6 mm×250 mm,5 μm）色谱柱,以水和乙腈为流动相进行梯度洗脱,梯度洗脱程序为：0~14 min,85%乙腈;14~16 min,85%~70%乙腈;16~28 min,70%乙腈;28~32 min,70%~85%乙腈,流速：1.0 mL/min,柱温：20℃,雾化管温度为30℃,漂移管温度为70℃,氮气压力为310.275 Pa。鼠李糖、阿拉伯糖、木糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖在3.202~184.828 μg范围内程良好的线性关系,8种糖的加标回收率在90.97%~105.07%之间,最低检测限（S/N=3）范围在6.976~1376.297 ng。该方法具有操作简便,分离效果好等特点。

A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH₂P-50-4E (4.6 mm×250 mm, 5 μm) column was used at 20℃, acetonitrile and water used as mobile phase, for gradient elusion with the flow rate of 1.0 mL/min. The ratio of organic solvent was controlled by a gradient elution procedure. The procedure was as follows: 85% acetonitrile in 0~14 min, 70%~80% acetonitrile 14~16 min, 70% acetonitrile 16~28 min, then 70%~80% acetonitrile 28~32 min. At last, the ratio of acetonitrile increased to 85%. With the use of evaporative light-scattering detector, the nebulization chamber temperature was 30℃, the drift tube temperature was 70℃ and nitrogen pressure was 310.275 Pa. The calibration curves of rhamnose, arabinose, xylose, fructose, mannose, glucose, sucrose and maltose showed good linearity in the range of 3.202~184.828 μg. The recoveries of fortified 8 kinds of sugar were 90.97%~105.07%, limit of detention was 6.976~1 376.297 ng. Results indicated that the method was simple, rapid and could effectively separate the target component.