关键词: 高效液相色谱, 蒸发光散射检测器, 茶叶, 单糖, 双糖

Abstract: A method for determination of monosaccharide and disaccharide in tea by HPLC-ELSD was developed. Shodex NH₂P-50-4E (4.6 mm×250 mm, 5 μm) column was used at 20℃, acetonitrile and water used as mobile phase, for gradient elusion with the flow rate of 1.0 mL/min. The ratio of organic solvent was controlled by a gradient elution procedure. The procedure was as follows: 85% acetonitrile in 0~14 min, 70%~80% acetonitrile 14~16 min, 70% acetonitrile 16~28 min, then 70%~80% acetonitrile 28~32 min. At last, the ratio of acetonitrile increased to 85%. With the use of evaporative light-scattering detector, the nebulization chamber temperature was 30℃, the drift tube temperature was 70℃ and nitrogen pressure was 310.275 Pa. The calibration curves of rhamnose, arabinose, xylose, fructose, mannose, glucose, sucrose and maltose showed good linearity in the range of 3.202~184.828 μg. The recoveries of fortified 8 kinds of sugar were 90.97%~105.07%, limit of detention was 6.976~1 376.297 ng. Results indicated that the method was simple, rapid and could effectively separate the target component.

Key words: HPLC, ELSD, tea, monosaccharides, disaccharides